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PMID: 18649184 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Trafficking dynamics of glycosylated pannexin 1 proteins.

Cell communication & adhesion ·Vol. 15 ·No. 1 ·2008-05-00 ·Pages 119-32

Boassa D, Qiu F, Dahl G, Sosinsky G

Abstract

Pannexins are mammalian orthologs of innexins and have a predicted topological folding pattern similar to that of connexins, except they are glycosylated. Rat pannexin 1 is glycosylated at N254 and this residue is important for plasma membrane targeting. Here we demonstrate that cell surface expression levels of the rat pannexin 1 N254Q mutant are rescued by coexpression with the wild-type protein. In paired Xenopus oocytes, the functional effect of this rescue is inconsequential; however, cell surface deglycosylation by PNGase F significantly enhanced functional gap junction formation. In mammalian cells, wild-type oligomers traffic at a slower rate than Myc-or tetracysteine domain-tagged versions, a behavior opposite to that of tagged connexins. The temporal differences of Panx1 trafficking correlate with spatial differences of intracellular localizations induced by Golgi blockage by Brefeldin-A or glycosylation prevention by tunicamycin. Therefore, Panx1 has kinetics and dynamics that make it unique to serve distinct functions separate from connexin-based channels.

MeSH Terms
Amino Acid Substitution Animals Cell Line Connexins/chemistry,genetics,metabolism Dogs Follow-Up Studies Gap Junctions/chemistry,metabolism Glycosylation Humans Kinetics Nerve Tissue Proteins/chemistry,genetics,metabolism Protein Structure, Tertiary Protein Transport/physiology Rats
Chemicals
Connexins Nerve Tissue Proteins pannexin 1, rat
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boassa Daniela
National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, La Jolla, California 92093-0608, USA. [email protected]
Qiu Feng
Dahl Gerhard
Sosinsky Gina
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Article Info
Journal
Cell communication & adhesion
Abbr.
Cell Commun Adhes
ISSN
1543-5180
DOI
10.1080/15419060802013885
Email
[email protected]
Published
2008-05-00
Pages
119-32
Language
English
Region
England
NLM ID
101096596
PMCID
PMC2528835
Subset
IM
Grants
NIGMS NIH HHS · R01 GM072881-02 · United States
NCRR NIH HHS · P41 RR004050-20 · United States
NIGMS NIH HHS · R01 GM065937-04 · United States
NCRR NIH HHS · P41 RR004050-178230 · United States
NIGMS NIH HHS · R01 GM065937-04S1 · United States
NIGMS NIH HHS · R01 GM048610 · United States
NCRR NIH HHS · RR04050 · United States
NIGMS NIH HHS · R01 GM072881-03 · United States
NCRR NIH HHS · P41 RR004050 · United States
NIGMS NIH HHS · R01 GM072881 · United States
NIGMS NIH HHS · R01 GM065937 · United States
NIGMS NIH HHS · GM072881 · United States
NIGMS NIH HHS · GM065937 · United States
NIGMS NIH HHS · GM48610 · United States
NIGMS NIH HHS · R01 GM048610-13A2 · United States
NIGMS NIH HHS · R01 GM072881-01 · United States
NIGMS NIH HHS · R01 GM072881-04 · United States
External Links
PubMed source DOI: 10.1080/15419060802013885 PMC Full Text
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