Interleukin-6 antisense oligonucleotides inhibit the growth of human myeloma cell lines.

The Journal of clinical investigation ·Vol. 88 ·No. 2 ·1991-08-00 ·Pages 696-9

Levy Y, Tsapis A, Brouet JC

Abstract

IL-6 has been shown to be a plasmacytoma growth factor in mice and is believed to play a key role in the development of human multiple myeloma. We investigated the IL-6 requirements for the growth of two human myeloma cell lines, U 266 and RPMI 8226. These cell lines secreted minute amounts of IL-6 (20 U/ml) and featured IL-6 mRNA. IL-6 receptors were detectable at the surface of malignant cells by immunofluorescence. Antibodies to IL-6 did not alter the proliferation of these myeloma cells. There was a dose-dependent decrease, however, in [3H]-thymidine uptake in the presence of IL-6 antisense (and not sense) oligodeoxynucleotides; in the presence of 20 microM IL-6 antisense, an 80 and 95% inhibition of the proliferation of U 266 and RPMI 8226 cells was observed, respectively. These results provide strong evidence for an IL-6 autocrine proliferation of myeloma cells which may occur via internal interaction between IL-6 and the IL-6 receptor.

MeSH Terms

Base Sequence Cell Division/drug effects Humans Interleukin-6/genetics,physiology Molecular Sequence Data Multiple Myeloma/etiology Oligonucleotides, Antisense/pharmacology Tumor Cells, Cultured

Chemicals

Interleukin-6 Oligonucleotides, Antisense

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Levy Y

Laboratory of Immunochemistry and Immunopathology, Institut National de la Santé et de la Recherche Médicale U 108, Hôpital Saint-Louis, Paris, France.

Tsapis A

Brouet J C

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Article Info

Journal

The Journal of clinical investigation

Abbr.

J Clin Invest

ISSN

0021-9738

DOI

10.1172/JCI115355

Published

1991-08-00

Pages

696-9

Language

English

Region

United States

NLM ID

7802877

PMCID

PMC295417

Subset

External Links

PubMed source DOI: 10.1172/JCI115355 PMC Full Text View journal details

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Interleukin-6 antisense oligonucleotides inhibit the growth of human myeloma cell lines.

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