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PMID: 18682978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

NbLRK1, a lectin-like receptor kinase protein of Nicotiana benthamiana, interacts with Phytophthora infestans INF1 elicitin and mediates INF1-induced cell death.

Planta ·Vol. 228 ·No. 6 ·2008-11-00 ·Pages 977-87

Kanzaki H, Saitoh H, Takahashi Y, Berberich T, Ito A, Kamoun S, Terauchi R

Abstract

Phytophthora infestans INF1 elicitin causes the hypersensitive response (HR) in Nicotiana benthamiana (Kamoun et al. in Plant Cell 10:1413-1425, 1998). To identify N. benthamiana proteins that interact with INF1, we carried out a yeast two-hybrid screen. This screen resulted in the isolation of a gene NbLRK1 coding for a novel lectin-like receptor kinase. NbLRK1 interacted with INF1 through its VIb kinase subdomain. Purified INF1 and NbLRK1 proteins also interacted in vitro. INF1 treatment of N. benthamiana leaves induced autophosphorylation of NbLRK1. Most importantly, virus-induced gene silencing (VIGS) of NbLRK1 delayed INF1-mediated HR in N. benthamiana. These data suggest that NbLRK1 is a component of the N. benthamiana protein complex that recognizes INF1 elicitor and transduces the HR signal.

MeSH Terms
Algal Proteins/metabolism Amino Acid Sequence Blotting, Northern Cell Membrane/enzymology,metabolism Gene Expression Genome, Plant Molecular Sequence Data Myelin Basic Protein/metabolism Phosphorylation Phytophthora infestans Plant Proteins/analysis,chemistry,genetics,metabolism Protein Kinases/analysis,chemistry,genetics,metabolism Protein Structure, Tertiary Proteins Sequence Alignment Tobacco/enzymology,metabolism Two-Hybrid System Techniques
Chemicals
Algal Proteins Myelin Basic Protein Plant Proteins Proteins elicitin, Phytophthora Protein Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kanzaki Hiroyuki
Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
Saitoh Hiromasa
Takahashi Yoshihiro
Berberich Thomas
Ito Akiko
Kamoun Sophien
Terauchi Ryohei
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-008-0797-y
Published
2008-11-00
Epub
2008-00-06
Pages
977-87
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-008-0797-y View journal details
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