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PMID: 18692475 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A protein domain-based interactome network for C. elegans early embryogenesis.

Cell ·Vol. 134 ·No. 3 ·2008-08-08 ·Pages 534-45

Boxem M, Maliga Z, Klitgord N, Li N, Lemmens I, Mana M, de Lichtervelde L, Mul JD, van de Peut D, Devos M, Simonis N, Yildirim MA, Cokol M, Kao HL, de Smet AS, Wang H, Schlaitz AL, Hao T, Milstein S, Fan C, Tipsword M, Drew K, Galli M, Rhrissorrakrai K, Drechsel D, Koller D, Roth FP, Iakoucheva LM, Dunker AK, Bonneau R, Gunsalus KC, Hill DE, Piano F, Tavernier J, van den Heuvel S, Hyman AA, Vidal M

Abstract

Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.

MeSH Terms
Animals Caenorhabditis elegans/embryology Cell Division Embryo, Nonmammalian/metabolism Embryonic Development Protein Interaction Domains and Motifs Protein Interaction Mapping Proteome Two-Hybrid System Techniques
Chemicals
Proteome
Authors & Affiliations
37 authors, click to expand affiliations / ORCID
Boxem Mike
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. [email protected]
Maliga Zoltan
Klitgord Niels
Li Na
Lemmens Irma
Mana Miyeko
de Lichtervelde Lorenzo
Mul Joram D
van de Peut Diederik
Devos Maxime
Simonis Nicolas
Yildirim Muhammed A
Cokol Murat
Kao Huey-Ling
de Smet Anne-Sophie
Wang Haidong
Schlaitz Anne-Lore
Hao Tong
Milstein Stuart
Fan Changyu
Tipsword Mike
Drew Kevin
Galli Matilde
Rhrissorrakrai Kahn
Drechsel David
Koller Daphne
Roth Frederick P
Iakoucheva Lilia M
Dunker A Keith
Bonneau Richard
Gunsalus Kristin C
Hill David E
Piano Fabio
Tavernier Jan
van den Heuvel Sander
Hyman Anthony A
Vidal Marc
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2008.07.009
Email
[email protected]
Published
2008-08-08
Pages
534-45
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2596478
Subset
IM
Grants
NCI NIH HHS · R21CA113711 · United States
NHGRI NIH HHS · R01 HG001715-06 · United States
NCI NIH HHS · R21 CA113711-01A2 · United States
NCI NIH HHS · R33CA81658 · United States
NCI NIH HHS · R33 CA105405-02 · United States
NHGRI NIH HHS · R01HG001715 · United States
NCI NIH HHS · R33 CA081658-04 · United States
NCRR NIH HHS · R21RR023114 · United States
NCI NIH HHS · P01 CA095281-01A10004 · United States
NCI NIH HHS · U54 CA011295 · United States
NCI NIH HHS · P01 CA095281 · United States
NCI NIH HHS · U54 CA112952-01 · United States
NCI NIH HHS · U54 CA112952-02 · United States
NCRR NIH HHS · R21 RR023114-02 · United States
NCRR NIH HHS · R21 RR023114 · United States
NCRR NIH HHS · R21 RR023114-01 · United States
NHGRI NIH HHS · R01 HG001715-07 · United States
NCI NIH HHS · CA95281 · United States
NCI NIH HHS · U54 CA112952-03 · United States
NCI NIH HHS · R33 CA105405-03 · United States
NHGRI NIH HHS · R01 HG001715 · United States
NCI NIH HHS · R33CA105405 · United States
NHGRI NIH HHS · R01 HG001715-09 · United States
NCI NIH HHS · R33 CA105405 · United States
NHGRI NIH HHS · R01 HG001715-10 · United States
NCI NIH HHS · U54 CA112952 · United States
NCI NIH HHS · R21 CA113711 · United States
NCI NIH HHS · R33 CA105405-01 · United States
NHGRI NIH HHS · R01 HG001715-07S1 · United States
NHGRI NIH HHS · R01 HG001715-08 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1016/j.cell.2008.07.009 PMC Full Text View journal details
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