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PMID: 18839199 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A new class of retroviral and satellite encoded small RNAs emanates from mammalian centromeres.

Chromosoma ·Vol. 118 ·No. 1 ·2009-02-00 ·Pages 113-25

Carone DM, Longo MS, Ferreri GC, Hall L, Harris M, Shook N, Bulazel KV, Carone BR, Obergfell C, O'Neill MJ, O'Neill RJ

Abstract

The transcriptional framework of the eukaryotic centromere core has been described in budding yeast and rice, but for most eukaryotes and all vertebrates it remains largely unknown. The lack of large pericentric repeats in the tammar wallaby has made it possible to map and identify the transcriptional units at the centromere in a mammalian species for the first time. We show that these transcriptional units, comprised of satellites and a retrovirus, are bound by centromere proteins and that they are the source of a novel class of small RNA. The endogenous retrovirus from which these small RNAs are derived is now known to be in the centromere domain of several vertebrate classes. The discovery of this new RNA form brings together several independent lines of evidence that point to a conserved retroviral-encoded processed RNA entity within eukaryotic centromeres.

MeSH Terms
Animals Cells, Cultured Centromere/genetics,physiology Chromosomes/genetics Chromosomes, Artificial, Bacterial Fibroblasts In Situ Hybridization, Fluorescence Mammals/genetics,metabolism Mice RNA, Satellite/genetics,metabolism Retroelements/genetics,physiology Retroviridae/genetics,physiology Transcription, Genetic
Chemicals
RNA, Satellite Retroelements
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Carone Dawn M
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA.
Longo Mark S
Ferreri Gianni C
Hall Laura
Harris Melissa
Shook Nicole
Bulazel Kira V
Carone Benjamin R
Obergfell Craig
O'Neill Michael J
O'Neill Rachel J
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
1432-0886
DOI
10.1007/s00412-008-0181-5
Published
2009-02-00
Epub
2008-00-07
Pages
113-25
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/s00412-008-0181-5 View journal details
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