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PMID: 188411 Published · ppublish English Comparative Study Journal Article

A comparison of bone matrix and tendon with particular reference to glycoprotein content.

The Biochemical journal ·Vol. 159 ·No. 3 ·1976-12-01 ·Pages 749-55

Herring GM

Abstract

Bone matrix and tendon are compared in terms of their carbohydrate and non-collagenous protein composition. The collagen content of both tissues was similar (90-91%), but bone matrix had at least three times as much sialic acid (0.28%) as tendon (0.08%). Smaller differences were found in the analysis of hexoses and hexosamines. After digestion with bacterial collagenase, about 9% of the total protein from both tissues was non-diffusible on dialysis, and this contained only 0.15% (bone) and 0.7% (tendon) of the original hydroxyproline; recovery of sialic acid was 86-87%. The collagenase-resistant soluble material amounted to about 9% (bone matrix) and 5% (tendon); the insoluble residues were 1 and 4% respectively. There were clear differences in the carbohydrate contents of the digests, but the amino acid compositions were similar. When the soluble digests were chromatographed on DEAE-cellulose, the elution profiles indicated the presence in each tissue of a variety of glycoproteins and a proteoglycan fraction, and showed clearly that an acidic glycoprotein corresponding to bone sialoprotein was not present in tendon.

MeSH Terms
Amino Acids/analysis Animals Bone and Bones/analysis Cattle Chromatography, DEAE-Cellulose Collagen/analysis Glycoproteins/analysis Hydroxyproline/analysis Microbial Collagenase Sialic Acids/analysis Sialoglycoproteins/analysis Tendons/analysis
Chemicals
Amino Acids Glycoproteins Sialic Acids Sialoglycoproteins Collagen Microbial Collagenase Hydroxyproline
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Herring G M
References (31)
31 references, click to expand
  1. The preparation of a bacterial collagenase containing negligible non-specific protease activity.
    Prep Biochem. 1975;5(3):229-45 PMID: 170602
  2. Glycoproteins of the connective tissue matrix.
    Int Rev Connect Tissue Res. 1976;7:251-322 PMID: 177379
  3. Isolation of a glycoprotein and proteodermatan sulphate from bovine achilles tendon by affinity chromatography on concanavalin A-Sepharose.
    Biochim Biophys Acta. 1975 Feb 27;379(2):444-55 PMID: 1122296
  4. Application of cetylpyridinium-complex elution methods to analysis of glycosaminoglycans of tendon.
    Biochim Biophys Acta. 1968 Jun 24;158(3):336-43 PMID: 4232353
  5. A direct photometric method for chloride in biological fluids, employing mercuric thiocyanate and perchloric acid.
    Clin Chem. 1966 Jan;12(1):1-17 PMID: 4285014
  6. The periodate oxidation of bovine bone sialoprotein, and some observations on its structure.
    Biochem J. 1969 Mar;111(5):621-7 PMID: 4306463
  7. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins.
    Biochemistry. 1971 Mar 16;10(6):988-94 PMID: 4323854
  8. Acidic structural proteins of connective tissue. Characterization of their heterogeneous nature.
    Eur J Biochem. 1971 Jun 11;20(3):426-31 PMID: 4325687
  9. Studies on the active site of clostripain. The specific inactivation by the chloromethyl ketone derived from -N-tosyl-L-lysine.
    J Biol Chem. 1971 Dec 25;246(24):7675-82 PMID: 4332560
  10. Recommendations concerning the use of bacterial collagenase for isolating collagen-associated molecules from tissues.
    Anal Biochem. 1973 May;53(1):313-6 PMID: 4351122
  11. Studies on bone matrix glycoproteins. Incorporation of (1-14C)glucosamine and plasma (14C)glycoprotein into rabbit cortical bone.
    Biochem J. 1973 Sep;136(1):125-34 PMID: 4359104
  12. Isolation and partial characterization of a glycoprotein from bovine cortical bone.
    Eur J Biochem. 1974 Jun 15;45(2):525-33 PMID: 4368850
  13. The isolation of soluble proteins, glycoproteins, and proteoglycans from bone.
    Prep Biochem. 1974;4(2):179-200 PMID: 4370482
  14. New sialic acids. Identification of N-glycoloyl-O-acetylneuraminic acids and N-acetyl-O-glycoloylneuraminic acids by improved methods for detection of N-acyl and O-acyl groups and by gas-liquid chromatography.
    Eur J Biochem. 1974 Dec 16;50(1):71-82 PMID: 4476281
  15. The isolation of glycoproteins from bovine achilles tendon and their interaction with collagen.
    Biochem J. 1972 Mar;127(1):179-86 PMID: 4627448
  16. Acidic structural proteins of connective tissue. I. Solubilization and preliminary chemical characterization.
    Biochim Biophys Acta. 1969 Nov 11;194(1):112-20 PMID: 5353121
  17. Intestinal blood flow and drug absorption from the rat jejunum.
    Life Sci. 1968 May 1;7(9):493-8 PMID: 5650605
  18. Carbohydrate content of bovine collagen preparations.
    Biochem J. 1968 Jul;108(4):587-91 PMID: 5667271
  19. The physical properties of a glycoprotein from bovine cortical bone (bone sialoprotein).
    Biochim Biophys Acta. 1965 Nov 1;101(3):327-35 PMID: 5862222
  20. A rapid procedure for the estimation of amino sugars on a micro scale.
    Anal Biochem. 1966 Apr;15(1):167-71 PMID: 5959422
  21. Studies on haem alpha-methenyl oxygenase. Isomeric structure of formylbiliverdin, a possible precursor of biliverdin.
    Biochem J. 1967 Jul;104(1):20-2 PMID: 6035512
  22. Some studies on the composition of bovine cortical-bone sialoprotein.
    Biochem J. 1967 Sep;104(3):705-15 PMID: 6049914
  23. The acid mucopolysaccharides of connective tissue.
    Biochim Biophys Acta. 1956 Sep;21(3):506-18 PMID: 13363957
  24. A micro-electrophoretic method.
    Nature. 1958 Mar 22;181(4612):839-40 PMID: 13526686
  25. Clostridium histolyticum collagenase: its purification and properties.
    Arch Biochem Biophys. 1958 Apr;74(2):465-75 PMID: 13534676
  26. Use of cellulose acetate and Ponceau S for electrophoretic serum protein analysis.
    Am J Med Technol. 1961 Jul-Aug;27:197-203 PMID: 13753260
  27. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.
    Biochem J. 1961 Nov;81:384-92 PMID: 13860975
  28. COMPARISON OF BOVINE BONE SIALOPROTEIN AND SERUM OROSOMUCOID.
    Nature. 1964 Feb 15;201:709 PMID: 14139700
  29. On the presence of sialic acid in certain glycoproteins and in gangliosides.
    Acta Soc Med Ups. 1952;57(3-4):230-41 PMID: 14943604
  30. The binding of hydrogen ions to an acidic glycoprotein from bovine cortical bone.
    Biochem J. 1967 Dec;105(3):1171-5 PMID: 16742543
  31. The binding of calcium and yttrium ions to a glycoprotein from bovine cortical bone.
    Biochem J. 1967 Dec;105(3):1177-85 PMID: 16742544
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1590749
Published
1976-12-01
Pages
749-55
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1164178
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1590749 PMC Full Text View journal details
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