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PMID: 188753 Published · ppublish English Journal Article

Banded karyotypes of H-4-IIE-C3 rat hepatoma cells grown in vitro.

In vitro ·Vol. 12 ·No. 9 ·1976-09-00 ·Pages 658-64

Mullen VT, Barnett CA

Abstract

Mitotic cells from the H-4-IIE-C3 rat hepatoma tissue culture line showed a range of 45 to 53 chromosomes per cell with 75% of the cells displaying a chromosome mumber between 49 and 52. Analysis of Wright's-Giemsa banded karyotypes of 22 cells revealed considerable cell to cell variation. Twenty-one structurally abnormal chromosomes were identified in these cells; the origin of nine of the 21 chromosomes were identified in these cells; the origin of nine of the 21 chromosomes could be determined. Of the structurally abnormal chromosomes detected, only one (M-1) occurred with a sufficiently high frequency to be of general use as a marker for these cells. This marker appears to be a Robertsonian translocation involving chromosome number 2 and chromosome number 10.

MeSH Terms
Animals Carcinoma, Hepatocellular/genetics Cell Line Chromosome Aberrations Chromosomes In Vitro Techniques Karyotyping Liver Neoplasms/genetics Male Rats
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mullen V T
Barnett C A
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19 references, click to expand
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02797467
Published
1976-09-00
Pages
658-64
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02797467
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