Expression of differentiated functions in hepatoma cell hybrids: reappearance of tyrosine aminotransferase inducibility after the loss of chromosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 3026-30

Weiss MC, Chaplain M

Abstract

HYBRIDS FROM A CROSS OF RAT HEPATOMA CELLS WITH DIPLOID EPITHELIAL CELLS FROM RAT LIVER HAVE BEEN STUDIED WITH RESPECT TO KARYOTYPE AND EXPRESSION OF TWO FUNCTIONS LIMITED TO THE HEPATOMA PARENT: high level of the enzyme tyrosine aminotransferase (EC 2.6.1.5; L-tyrosine:2-oxoglutarate aminotransferase) and its inducibility with steroid hormones. The hybrids that contain the complete chromosomal complements from both parents show low enzyme activity and no inducibility. One hybrid clone, and all of its derivatives, which have lost 30-40% of the chromosomes initially present, show enzyme inducibility. Induction of tyrosine aminotransferase in the hepatoma and hybrid cells responds similarly to inhibition by cycloheximide and actinomycin D, and to steroid concentration. The enzymes from induced and noninduced hepatoma cells and from induced hybrid cells are similar in heat sensitivity and intracellular distribution; those from noninduced hybrid and diploid rat epithelial cells are different.

MeSH Terms

Animals Carcinoma, Hepatocellular/enzymology Cell Fusion Cell Line Cells, Cultured Chromosomes Clone Cells Crosses, Genetic Cycloheximide/pharmacology Dactinomycin/pharmacology Depression, Chemical Dexamethasone/pharmacology Enzyme Activation/drug effects Enzyme Induction/drug effects Epithelial Cells Epithelium/enzymology Hot Temperature Hybrid Cells/enzymology In Vitro Techniques Karyotyping Liver/enzymology Liver Neoplasms Rats Tyrosine Transaminase/antagonists & inhibitors,metabolism

Chemicals

Dactinomycin Dexamethasone Cycloheximide Tyrosine Transaminase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Weiss M C

Chaplain M

References (19)

19 references, click to expand

Induction of tyrosine alpha-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line.
Proc Natl Acad Sci U S A. 1966 Jul;56(1):296-303 PMID: 4381784
Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA.
Science. 1969 Dec 19;166(3912):1474-80 PMID: 17655040
Regulation of pigment synthesis in mammalian cells, as studied by somatic hybridization.
Proc Natl Acad Sci U S A. 1966 Nov;56(5):1437-40 PMID: 5230304
Properties of a hybrid between lines sensitive and insensitive to contact inhibition of cell division.
J Cell Physiol. 1968 Feb;71(1):105-7 PMID: 5663109
Recombination and segregation in somatic cell hybrids.
Nature. 1969 Jul 12;223(5202):152-5 PMID: 4307226
Soluble and mitochondrial forms of tyrosine aminotransferase. Relationship to human tyrosinemia.
Biochemistry. 1969 Feb;8(2):615-22 PMID: 4389443
Suppression of malignancy by cell fusion.
Nature. 1969 Jul 26;223(5204):363-8 PMID: 5387828
Control of tyrosine aminotransferase synthesis in tissue culture by a factor in serum.
Proc Natl Acad Sci U S A. 1969 Oct;64(2):723-30 PMID: 4391023
Regulation of protein synthesis in HeLa cells: translation at elevated temperatures.
J Mol Biol. 1969 Jan;39(2):315-33 PMID: 5362672
Mapping of a human genetic regulator element by somatic cell genetic analysis.
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1220-7 PMID: 4920091
Superinduction of tyrosine aminotransferase by actinomycin D in rat hepatoma (HTC) cells.
J Mol Biol. 1970 Dec 14;54(2):159-75 PMID: 4395444
Expression of differentiated functions in hepatoma cell hybrids. I. Tyrosine aminotransferase in hepatoma-fibroblast hybrids.
Proc Natl Acad Sci U S A. 1971 Jan;68(1):127-31 PMID: 4395684
Purification, properties, and identity of liver mitochondrial tyrosine aminotransferase.
J Biol Chem. 1971 May 25;246(10):3234-40 PMID: 4396841
Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture.
J Mol Biol. 1968 Jul 28;35(2):291-301 PMID: 4398833
Expression of tyrosine aminotransferase activity in somatic-cell heterokaryons: evidence for negative control of enzyme expression.
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2589-93 PMID: 4109409
A transplantable bile-secreting hepatocellular carcinoma in the rat.
J Natl Cancer Inst. 1961 Apr;26:891-9 PMID: 13740982
Induction of tyrosine-alpha-ketoglutarate transaminase in rat liver. IV. Evidence for an increase in the rate of enzyme synthesis.
J Biol Chem. 1962 Nov;237:3495-8 PMID: 14031971
HEPATOMAS IN TISSUE CULTURE COMPARED WITH ADAPTING LIVER IN VIVO.
Natl Cancer Inst Monogr. 1964 Apr;13:229-45 PMID: 14143233
Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes.
J Mol Biol. 1966 Sep;20(1):145-82 PMID: 5970657

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.12.3026

Published

1971-12-00

Pages

3026-30

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389583

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.12.3026 PMC Full Text View journal details

Analysis Services

Expression of differentiated functions in hepatoma cell hybrids: reappearance of tyrosine aminotransferase inducibility after the loss of chromosomes.

Contact

WeChat Official Account