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PMID: 189072 Published · ppublish English Comparative Study Journal Article

Complexity and polyadenylic acid content of visna virus 60-70S RNA.

Journal of virology ·Vol. 21 ·No. 1 ·1977-01-00 ·Pages 386-95

Vigne R, Brahic M, Filippi P, Tamalet J

Abstract

The genomic complexity of visna virus was measured by quantitative analysis of 18 RNase T1-resistant oligonucleotides from 60-70S RNA. T1-resistant oligonucleotides were separated by two-dimensional polyacrylamide gel electrophoresis. Visna virus had a genomic complexity of 3.6 X 10(6) daltons, very close to the size of a single 30-40S RNA subunit. It was therefore concluded that the visna virus genome is largely polyploid. Visna virus 60-70S RNA polyadenylic acid segment was purified by T1 RNase digestion followed by oligodeoxythymidylic acid-cellulose column chromatography. It contained over 99% AMP and had a size of about 200 nucleotides. The binding capacities on oligodeoxythymidylic acid-cellulose of native 60-70S RNA and purified 30-40S RNA subunits were examined. It was concluded that two out of three intact subunits contain a polyadenylic acid segment.

MeSH Terms
Avian Sarcoma Viruses/analysis Base Sequence Molecular Weight Oligonucleotides/analysis Poly A/analysis RNA Viruses/analysis RNA, Viral/analysis Ribonucleases/metabolism Visna-maedi virus/analysis
Chemicals
Oligonucleotides RNA, Viral Poly A Ribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vigne R
Brahic M
Filippi P
Tamalet J
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27 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.21.1.386-395.1977
Published
1977-01-00
Pages
386-95
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353825
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.21.1.386-395.1977 PMC Full Text View journal details
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