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PMID: 18927509 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14.

Cell cycle (Georgetown, Tex.) ·Vol. 7 ·No. 20 ·2008-10-00 ·Pages 3262-72

Rahal R, Amon A

Abstract

Exit from mitosis in Saccharomyces cerevisiae is triggered by activation of the phosphatase Cdc14. Throughout interphase and early mitosis, Cdc14 is sequestered in the nucleolus by its inhibitor Cfi1/Net1. In anaphase, the Cdc Fourteen Early Anaphase Release (FEAR) network and the Mitotic Exit Network (MEN) coordinately trigger the release of Cdc14 from the nucleolus. Here we show that the FEAR network component Cdc5 physically associates with two other members of the pathway, the Separase Esp1 and the Esp1-binding protein Slk19. Furthermore, we find that Cdc5 physically interacts with Cdc14 and that this association is mediated by Cdc5's Polo-box domain, a phospho-serine/phosphothreonine binding domain. Finally, we present evidence that the Cdc5-Cdc14 association is direct, further supporting the central role of Cdc5 in Cdc14 localization.

MeSH Terms
Cell Cycle Proteins/genetics,metabolism Cell Nucleolus/metabolism Endopeptidases/genetics,metabolism Microtubule-Associated Proteins/genetics,metabolism Mitosis/physiology Protein Binding Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases Protein Structure, Tertiary Protein Tyrosine Phosphatases/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Separase Signal Transduction/physiology
Chemicals
CDC14 protein, S cerevisiae Cell Cycle Proteins Microtubule-Associated Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Slk19 protein, S cerevisiae Protein Kinases Protein Serine-Threonine Kinases CDC5 protein, S cerevisiae Protein Tyrosine Phosphatases Endopeptidases ESP1 protein, S cerevisiae Separase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rahal Rami
David H Koch Institute for Integrative Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
Amon Angelika
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Article Info
Journal
Cell cycle (Georgetown, Tex.)
Abbr.
Cell Cycle
ISSN
1551-4005
DOI
10.4161/cc.7.20.6852
Published
2008-10-00
Epub
2008-00-25
Pages
3262-72
Language
English
Region
United States
NLM ID
101137841
PMCID
PMC2665935
Subset
IM
Grants
NIGMS NIH HHS · R01 GM056800-13 · United States
NIGMS NIH HHS · R29 GM056800 · United States
NIGMS NIH HHS · R01 GM056800 · United States
NIGMS NIH HHS · GM 56800 · United States
Howard Hughes Medical Institute · United States
External Links
PubMed source DOI: 10.4161/cc.7.20.6852 PMC Full Text View journal details
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