Home LiteratureArticle Details
PMID: 18929664 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation.

Methods (San Diego, Calif.) ·Vol. 46 ·No. 3 ·2008-11-00 ·Pages 177-82

Betzenhauser MJ, Wagner LE, Won JH, Yule DI

Abstract

Inositol 1,4,5-trisphosphate receptors (InsP3R) are a family of ubiquitously expressed intracellular Ca2+ channels. Isoform-specific properties of the three family members may play a prominent role in defining the rich diversity of the spatial and temporal characteristics of intracellular Ca2+ signals. Studying the properties of the particular family members is complicated because individual receptor isoforms are typically never expressed in isolation. In this article, we discuss strategies for studying Ca2+ release through individual InsP3R family members with particular reference to methods applicable following expression of recombinant InsP3R and mutant constructs in the DT40-3KO cell line, an unambiguously null InsP3R expression system.

MeSH Terms
Animals Calcium/metabolism Calcium Signaling/physiology Cell Line, Tumor Chickens Fura-2/analogs & derivatives,metabolism Inositol 1,4,5-Trisphosphate Receptors/genetics,physiology Permeability Photolysis Protein Isoforms/metabolism Rats
Chemicals
Inositol 1,4,5-Trisphosphate Receptors Protein Isoforms 2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid Calcium Fura-2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Betzenhauser Matthew J
Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA.
Wagner Larry E
Won Jong Hak
Yule David I
References (45)
45 references, click to expand
  1. Lateral inhibition of inositol 1,4,5-trisphosphate receptors by cytosolic Ca(2+).
    Curr Biol. 1999 Oct 7;9(19):1115-8 PMID: 10531009
  2. The type II inositol 1,4,5-trisphosphate receptor can trigger Ca2+ waves in rat hepatocytes.
    Gastroenterology. 2002 Apr;122(4):1088-100 PMID: 11910359
  3. Ca(2+)-sensor region of IP(3) receptor controls intracellular Ca(2+) signaling.
    EMBO J. 2001 Apr 2;20(7):1674-80 PMID: 11285231
  4. Reduced IP3 sensitivity of IP3 receptor in Purkinje neurons.
    Neuroreport. 2001 Aug 28;12(12):2647-51 PMID: 11522941
  5. Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum.
    Nat Cell Biol. 2003 May;5(5):440-6 PMID: 12717445
  6. Phosphorylation of type-1 inositol 1,4,5-trisphosphate receptors by cyclic nucleotide-dependent protein kinases: a mutational analysis of the functionally important sites in the S2+ and S2- splice variants.
    J Biol Chem. 2003 Nov 14;278(46):45811-7 PMID: 12939273
  7. Agonist-induced Ca2+ entry determined by inositol 1,4,5-trisphosphate recognition.
    Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2323-7 PMID: 14983008
  8. Distinct roles of inositol 1,4,5-trisphosphate receptor types 1 and 3 in Ca2+ signaling.
    J Biol Chem. 2004 Mar 19;279(12):11967-75 PMID: 14707143
  9. Inositol 1,4,5-trisphosphate receptors as signal integrators.
    Annu Rev Biochem. 2004;73:437-65 PMID: 15189149
  10. Functional analysis of the green fluorescent protein-tagged inositol 1,4,5-trisphosphate receptor type 3 in Ca(2+) release and entry in DT40 B lymphocytes.
    Biochem J. 2004 Sep 15;382(Pt 3):793-801 PMID: 15175012
  11. Functional consequences of phosphomimetic mutations at key cAMP-dependent protein kinase phosphorylation sites in the type 1 inositol 1,4,5-trisphosphate receptor.
    J Biol Chem. 2004 Oct 29;279(44):46242-52 PMID: 15308649
  12. Solubilization, purification, and characterization of an inositol trisphosphate receptor.
    J Biol Chem. 1988 Jan 25;263(3):1530-4 PMID: 2826483
  13. Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400.
    Nature. 1989 Nov 2;342(6245):32-8 PMID: 2554142
  14. Quantification of intraluminal free [Ca] in the agonist-sensitive internal calcium store using compartmentalized fluorescent indicators: some considerations.
    Cell Calcium. 1996 Sep;20(3):235-42 PMID: 8894270
  15. Isoform diversity of the inositol trisphosphate receptor in cell types of mouse origin.
    Biochem J. 1997 Mar 1;322 ( Pt 2):575-83 PMID: 9065779
  16. T cells deficient in inositol 1,4,5-trisphosphate receptor are resistant to apoptosis.
    Mol Cell Biol. 1997 Jun;17(6):3005-12 PMID: 9154798
  17. Genetic evidence for involvement of type 1, type 2 and type 3 inositol 1,4,5-trisphosphate receptors in signal transduction through the B-cell antigen receptor.
    EMBO J. 1997 Jun 2;16(11):3078-88 PMID: 9214625
  18. Minimal requirements for calcium oscillations driven by the IP3 receptor.
    EMBO J. 1997 Jun 16;16(12):3533-43 PMID: 9218795
  19. Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18.
    Biochem Biophys Res Commun. 1997 Nov 7;240(1):189-95 PMID: 9367908
  20. Phosphorylation of inositol 1,4,5-trisphosphate receptors by cAMP-dependent protein kinase. Type I, II, and III receptors are differentially susceptible to phosphorylation and are phosphorylated in intact cells.
    J Biol Chem. 1998 Mar 6;273(10):5670-7 PMID: 9488697
  21. Type III InsP3 receptor channel stays open in the presence of increased calcium.
    Nature. 1998 Nov 5;396(6706):81-4 PMID: 9817204
  22. Encoding of Ca2+ signals by differential expression of IP3 receptor subtypes.
    EMBO J. 1999 Mar 1;18(5):1303-8 PMID: 10064596
  23. Molecular properties of inositol 1,4,5-trisphosphate receptors.
    Cell Calcium. 1999 Mar;25(3):247-64 PMID: 10378086
  24. Molecular cloning of mouse type 2 and type 3 inositol 1,4,5-trisphosphate receptors and identification of a novel type 2 receptor splice variant.
    J Biol Chem. 2005 Mar 18;280(11):10305-17 PMID: 15632133
  25. The type III inositol 1,4,5-trisphosphate receptor preferentially transmits apoptotic Ca2+ signals into mitochondria.
    J Biol Chem. 2005 Dec 9;280(49):40892-900 PMID: 16192275
  26. Measurement of free [Ca2+] changes in agonist-sensitive internal stores using compartmentalized fluorescent indicators.
    Methods Mol Biol. 2006;312:229-47 PMID: 16422202
  27. ATP binding to a unique site in the type-1 S2- inositol 1,4,5-trisphosphate receptor defines susceptibility to phosphorylation by protein kinase A.
    J Biol Chem. 2006 Jun 23;281(25):17410-9 PMID: 16621795
  28. Protein kinase C phosphorylates the inositol 1,4,5-trisphosphate receptor type 2 and decreases the mobilization of Ca2+in pancreatoma AR4-2J cells.
    J Endocrinol. 2007 Mar;192(3):659-68 PMID: 17332533
  29. Rapid functional assays of intracellular Ca2+ channels.
    Nat Protoc. 2006;1(1):259-63 PMID: 17406242
  30. Inositol trisphosphate receptor Ca2+ release channels.
    Physiol Rev. 2007 Apr;87(2):593-658 PMID: 17429043
  31. Protein kinase A increases the binding affinity and the Ca2+ release activity of the inositol 1,4,5-trisphosphate receptor type 3 in RINm5F cells.
    Biol Cell. 2007 Jul;99(7):379-88 PMID: 17373911
  32. Cell membrane permeable esters of D-myo-inositol 1,4,5-trisphosphate.
    Cell Calcium. 2007 Sep;42(3):291-301 PMID: 17307252
  33. Ca2+ release dynamics in parotid and pancreatic exocrine acinar cells evoked by spatially limited flash photolysis.
    Am J Physiol Gastrointest Liver Physiol. 2007 Dec;293(6):G1166-77 PMID: 17901163
  34. Inositol 1,4,5-trisphosphate receptors: distinct neuronal and nonneuronal forms derived by alternative splicing differ in phosphorylation.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2951-5 PMID: 1849282
  35. Structure of a novel InsP3 receptor.
    EMBO J. 1991 Nov;10(11):3199-206 PMID: 1655411
  36. Three additional inositol 1,4,5-trisphosphate receptors: molecular cloning and differential localization in brain and peripheral tissues.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4265-9 PMID: 1374893
  37. Technique for in situ measurement of calcium in intracellular inositol 1,4,5-trisphosphate-sensitive stores using the fluorescent indicator mag-fura-2.
    Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2598-602 PMID: 8464866
  38. Functional heterogeneity of calcium release by inositol trisphosphate in single Purkinje neurones, cultured cerebellar astrocytes, and peripheral tissues.
    Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4976-80 PMID: 8506344
  39. Primary structure, ligand binding, and localization of the human type 3 inositol 1,4,5-trisphosphate receptor expressed in intestinal epithelium.
    J Biol Chem. 1994 Jan 14;269(2):1222-30 PMID: 8288584
  40. Determination of relative amounts of inositol trisphosphate receptor mRNA isoforms by ratio polymerase chain reaction.
    J Biol Chem. 1994 Aug 26;269(34):21691-8 PMID: 8063813
  41. Heterogeneity of channel density in inositol-1,4,5-trisphosphate-sensitive Ca2+ stores.
    Nature. 1994 Dec 22-29;372(6508):791-4 PMID: 7997268
  42. Type I, II, and III inositol 1,4,5-trisphosphate receptors are unequally susceptible to down-regulation and are expressed in markedly different proportions in different cell types.
    J Biol Chem. 1995 May 12;270(19):11678-83 PMID: 7744807
  43. Fast activation and inactivation of inositol trisphosphate-evoked Ca2+ release in rat cerebellar Purkinje neurones.
    J Physiol. 1995 Sep 1;487 ( Pt 2):343-58 PMID: 8558468
  44. Imaging of intracellular calcium stores in individual permeabilized pancreatic acinar cells. Apparent homogeneous cellular distribution of inositol 1,4,5-trisphosphate-sensitive stores in permeabilized pancreatic acinar cells.
    J Biol Chem. 1996 Mar 1;271(9):4999-5006 PMID: 8617776
  45. Differential modulation of inositol 1,4,5-trisphosphate receptor type 1 and type 3 by ATP.
    Cell Calcium. 2000 May;27(5):257-67 PMID: 10859592
Article Info
Journal
Methods (San Diego, Calif.)
Abbr.
Methods
ISSN
1095-9130
DOI
10.1016/j.ymeth.2008.09.014
Published
2008-11-00
Epub
2008-00-16
Pages
177-82
Language
English
Region
United States
NLM ID
9426302
PMCID
PMC3069621
Subset
IM
Grants
NIDCR NIH HHS · R01 DE014756 · United States
NIDCR NIH HHS · T32-DE07202 · United States
NIDDK NIH HHS · R01 DK054568 · United States
NIDCR NIH HHS · R56 DE014756 · United States
NIDDK NIH HHS · R01-DK54568 · United States
NIDCR NIH HHS · R01/R56-DE14756 · United States
NIDCR NIH HHS · R01-DE16999 · United States
NIDDK NIH HHS · R01 DK054568-09 · United States
NIDCR NIH HHS · R01 DE016999 · United States
NIDCR NIH HHS · R01 DE014756-08 · United States
External Links
PubMed source DOI: 10.1016/j.ymeth.2008.09.014 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2