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PMID: 1895381 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Predominant binding of Theiler's viruses to a 34-kilodalton receptor protein on susceptible cell lines.

Journal of virology ·Vol. 65 ·No. 10 ·1991-10-00 ·Pages 5244-9

Kilpatrick DR, Lipton HL

Abstract

Western immunoblots of BHK-21 cell lysates probed with the highly virulent GDVII and the less virulent BeAn strains of Theiler's murine encephalomyelitis virus (TMEV) revealed predominant binding to a 34-kDa membrane protein and much lower levels of binding to 100- and 18-kDa membrane proteins. Complete inhibition of virus binding to both the 34- and 18-kDa membrane species by excess unlabeled TMEV demonstrated specificity of binding. Virus binding was also blocked by wheat germ agglutinin, which specifically binds to sialic acid residues and blocks TMEV binding to whole BHK-21 cells. Radiolabeled TMEV also bound to 100-, 34-, and 18-kDa membrane proteins expressed on other TMEV permissive cell lines but not on the nonpermissive cell lines tested. These data suggest that a 34-kDa cellular protein may be the primary determinant of susceptibility to TMEV infection by mediating the binding of GDVII and BeAn viruses to susceptible cells.

MeSH Terms
Animals Autoradiography Binding, Competitive Cell Line Cell Membrane/physiology Cricetinae Electrophoresis, Polyacrylamide Gel Maus Elberfeld virus/physiology Methionine/metabolism Molecular Weight Phosphates/metabolism Phosphorus Radioisotopes Receptors, Virus/biosynthesis,isolation & purification,physiology Sulfur Radioisotopes
Chemicals
Phosphates Phosphorus Radioisotopes Receptors, Virus Sulfur Radioisotopes Methionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kilpatrick D R
Department of Neurology, University of Colorado Health Sciences Center, Denver 80262.
Lipton H L
References (31)
31 references, click to expand
  1. Genetic resistance to mouse hepatitis virus correlates with absence of virus-binding activity on target tissues.
    J Virol. 1987 Jan;61(1):185-9 PMID: 3023696
  2. Comparison of the binding characteristics to BHK-21 cells of viruses representing the two Theiler's virus neurovirulence groups.
    Virology. 1991 May;182(1):365-70 PMID: 2024473
  3. Three-dimensional model of the capsid proteins of two biologically different Theiler virus strains: clustering of amino acid difference identifies possible locations of immunogenic sites on the virion.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4496-500 PMID: 2454472
  4. Class II-restricted T cell responses in Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease. I. Cross-specificity among TMEV substrains and related picornaviruses, but not myelin proteins.
    J Immunol. 1987 Jun 1;138(11):3776-84 PMID: 2438327
  5. cDNA cloning reveals that the major group rhinovirus receptor on HeLa cells is intercellular adhesion molecule 1.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):4907-11 PMID: 2544880
  6. The spleen focus-forming virus envelope glycoprotein is defective in oligomerization.
    J Biol Chem. 1989 Jun 25;264(18):10732-7 PMID: 2543680
  7. Effects of deletion of the cytoplasmic domain upon surface expression and membrane stability of a viral envelope glycoprotein.
    J Biol Chem. 1987 Nov 25;262(33):16116-21 PMID: 3680244
  8. The 5' noncoding sequences from a less virulent Theiler's virus dramatically attenuate GDVII neurovirulence.
    J Virol. 1991 Aug;65(8):4370-7 PMID: 2072455
  9. The pathogenesis of Theiler's GD VII encephalomyelitis virus infection in mice as studied by immunofluorescent technique and infectivity titrations.
    J Immunol. 1967 Jan;98(1):46-55 PMID: 4289554
  10. The major and minor group receptor families contain all but one human rhinovirus serotype.
    Virology. 1991 Feb;180(2):814-7 PMID: 1846502
  11. Polyomavirus replication in mice: influences of VP1 type and route of inoculation.
    J Virol. 1991 Jan;65(1):342-9 PMID: 1845895
  12. Determinants of persistence and demyelination of the DA strain of Theiler's virus are found only in the VP1 gene.
    J Virol. 1991 Mar;65(3):1616-8 PMID: 1844214
  13. Macrophage and T cell-line tropisms of HIV-1 are determined by specific regions of the envelope gp120 gene.
    Nature. 1991 Jan 10;349(6305):167-9 PMID: 1986308
  14. Protein blot analysis of virus receptors: identification and characterization of the Sendai virus receptor.
    Biochim Biophys Acta. 1986 Mar 27;856(1):19-26 PMID: 3006771
  15. The antibody response in Theiler's virus infection: new perspectives on multiple sclerosis.
    Prog Med Virol. 1987;34:156-79 PMID: 2821576
  16. The atomic structure of Mengo virus at 3.0 A resolution.
    Science. 1987 Jan 9;235(4785):182-91 PMID: 3026048
  17. Two amino acids, Phe 16 and Ala 776, on the polyprotein are most likely to be responsible for the diabetogenicity of encephalomyocarditis virus.
    J Gen Virol. 1990 Mar;71 ( Pt 3):639-45 PMID: 1690262
  18. Identification of a putative cell receptor for human cytomegalovirus.
    Virology. 1990 Jun;176(2):337-45 PMID: 1971735
  19. Genetic mapping of the ability of Theiler's virus to persist and demyelinate.
    J Virol. 1990 Sep;64(9):4252-7 PMID: 2166814
  20. Neurovirulence determinants of genetically engineered Theiler viruses.
    Proc Natl Acad Sci U S A. 1990 Jun;87(11):4125-9 PMID: 2161533
  21. The human cytomegalovirus receptor on fibroblasts is a 30-kilodalton membrane protein.
    J Virol. 1990 Jun;64(6):2484-90 PMID: 2159525
  22. Structural refinement and analysis of Mengo virus.
    J Mol Biol. 1990 Feb 20;211(4):803-44 PMID: 2156078
  23. Genomic regions of neurovirulence and attenuation in Theiler murine encephalomyelitis virus.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):978-82 PMID: 2153981
  24. A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus.
    Virology. 1990 Sep;178(1):161-5 PMID: 2389550
  25. Binding of 125I-labeled reovirus to cell surface receptors.
    Virology. 1984 Feb;133(1):46-55 PMID: 6702106
  26. Differential sensitivity of human, avian, and equine influenza A viruses to a glycoprotein inhibitor of infection: selection of receptor specific variants.
    Virology. 1983 Dec;131(2):394-408 PMID: 6197808
  27. A sialoglycopeptide from human erythrocytes with receptor-like properties for encephalomyocarditis and influenza viruses.
    J Gen Virol. 1983 May;64(Pt 5):1137-48 PMID: 6302211
  28. Analysis of genetic variation in Theiler's virus during persistent infection in the mouse central nervous system.
    Virology. 1983 Jul 15;128(1):16-32 PMID: 6192587
  29. The growth of four human and animal enteroviruses in the central nervous systems of mice.
    J Neuropathol Exp Neurol. 1981 May;40(3):258-70 PMID: 6260909
  30. Characterization of the TO strains of Theiler's mouse encephalomyelitis viruses.
    Infect Immun. 1978 Jun;20(3):869-72 PMID: 208981
  31. Single amino-acid changes in HIV envelope affect viral tropism and receptor binding.
    Nature. 1989 Aug 17;340(6234):571-4 PMID: 2475780
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.10.5244-5249.1991
Published
1991-10-00
Pages
5244-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249003
Subset
IM
Grants
CSR NIH HHS · BRG-05357 · United States
NINDS NIH HHS · NS21913 · United States
NINDS NIH HHS · NS23349 · United States
External Links
PubMed source DOI: 10.1128/JVI.65.10.5244-5249.1991 PMC Full Text View journal details
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