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PMID: 2159525 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The human cytomegalovirus receptor on fibroblasts is a 30-kilodalton membrane protein.

Journal of virology ·Vol. 64 ·No. 6 ·1990-06-00 ·Pages 2484-90

Taylor HP, Cooper NR

Abstract

Previous studies have demonstrated that human cytomegalovirus (HCMV) binding to human foreskin fibroblasts (HFF) is mediated by a single type of molecule, likely a glycoprotein, which serves as a specific receptor for the virus. In the present experiments, HCMV was found to bind to an HFF membrane protein with an approximate molecular mass of 30 kilodaltons (kDa); weak binding to 28- and 92-kDa membrane components was also observed. Binding was specific, as it was inhibited by excess unlabeled HCMV. Radiolabeled HCMV also bound selectively to Raji and Daudi lymphoblastoid cell membrane proteins of the same molecular masses. The 30-kDa radiolabeled HFF membrane protein bound to HCMV in solution; this binding was also specific, as it was blocked by an excess of HCMV. These data suggest that a membrane protein with a molecular mass of approximately 30 kDa mediates HCMV binding to several cell types.

MeSH Terms
Cell Line Cell Membrane/metabolism Cells, Cultured Cytomegalovirus/physiology Electrophoresis, Polyacrylamide Gel Fibroblasts/metabolism Humans Immunoblotting Membrane Proteins/isolation & purification Molecular Weight Receptors, Cell Surface/isolation & purification,metabolism Receptors, Virus
Chemicals
Membrane Proteins Receptors, Cell Surface Receptors, Virus cytomegalovirus receptor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Taylor H P
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cooper N R
References (48)
48 references, click to expand
  1. Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):6134-8 PMID: 6091137
  2. Beta 2 microglobulin binds to the tegument of cytomegalovirus: an immunogold study.
    J Gen Virol. 1989 Aug;70 ( Pt 8):2179-84 PMID: 2549187
  3. T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):767-8 PMID: 6083454
  4. gp140, the C3d receptor of human B lymphocytes, is also the Epstein-Barr virus receptor.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1490-3 PMID: 2983347
  5. Identification and characterization of the Epstein-Barr virus receptor on human B lymphocytes and its relationship to the C3d complement receptor (CR2).
    J Virol. 1985 Aug;55(2):347-51 PMID: 2410629
  6. Purification of the Epstein-Barr virus/C3d complement receptor of human B lymphocytes: antigenic and functional properties of the purified protein.
    J Virol. 1986 May;58(2):709-12 PMID: 2422399
  7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  8. Plaque assay of cytomegalovirus strains of human origin.
    Proc Soc Exp Biol Med. 1970 Nov;135(2):253-8 PMID: 4320336
  9. Epidemiology of cytomegalovirus infection after transplantation and immunosuppression.
    J Infect Dis. 1975 Oct;132(4):421-33 PMID: 171315
  10. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  11. Human cytomegalovirus: glycoproteins associated with virions and dense bodies.
    J Virol. 1976 Aug;19(2):594-609 PMID: 183016
  12. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
    J Biol Chem. 1977 Sep 25;252(18):6510-5 PMID: 893422
  13. Human cytomegalovirus DNA. I. Molecular weight and infectivity.
    J Virol. 1978 Jun;26(3):813-6 PMID: 209219
  14. Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):10100-3 PMID: 2557618
  15. Soluble recombinant CR2 (CD21) inhibits Epstein-Barr virus infection.
    J Virol. 1990 Mar;64(3):1348-52 PMID: 2154612
  16. Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids.
    Proc Soc Exp Biol Med. 1956 Jun;92(2):418-24 PMID: 13350367
  17. Chemical, physical-chemical, and immunological properties of papain-solubilized human transplatation antigens.
    Biochemistry. 1979 May 29;18(11):2218-26 PMID: 444449
  18. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  19. Role of beta2-microglobulin in the intracellular processing of HLA antigens.
    Biochemistry. 1981 Aug 4;20(16):4523-30 PMID: 6170312
  20. Neutralization of Epstein-Barr virus by nonimmune human serum. Role of cross-reacting antibody to herpes simplex virus and complement.
    J Clin Invest. 1982 Nov;70(5):1081-91 PMID: 6290536
  21. Congenital and perinatal cytomegalovirus infections.
    Semin Perinatol. 1983 Jan;7(1):31-42 PMID: 6302912
  22. Cytomegalovirus infection of human blood cells.
    J Infect Dis. 1984 Feb;149(2):207-14 PMID: 6321607
  23. Binding of 125I-labeled reovirus to cell surface receptors.
    Virology. 1984 Feb;133(1):46-55 PMID: 6702106
  24. Characterization of saturable binding sites for rabies virus.
    J Virol. 1984 Jun;50(3):691-7 PMID: 6726888
  25. Herpesvirus infections in the acquired immune deficiency syndrome.
    JAMA. 1984 Jul 6;252(1):72-7 PMID: 6328055
  26. Many rhinovirus serotypes share the same cellular receptor.
    J Virol. 1984 Aug;51(2):340-5 PMID: 6086949
  27. Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4510-4 PMID: 6087328
  28. Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of a nondenaturing ("native" gel) system of polyacrylamide gel electrophoresis coupled with Western blotting.
    J Virol. 1986 Oct;60(1):157-66 PMID: 2427745
  29. Cytomegalovirus strain AD169 binds beta 2 microglobulin in vitro after release from cells.
    J Gen Virol. 1987 Mar;68 ( Pt 3):777-84 PMID: 3029304
  30. Cytomegalovirus in urine specimens has host beta 2 microglobulin bound to the viral envelope: a mechanism of evading the host immune response?
    J Gen Virol. 1987 Mar;68 ( Pt 3):785-92 PMID: 3029305
  31. Beta 2 microglobulin enhances the infectivity of cytomegalovirus and when bound to the virus enables class I HLA molecules to be used as a virus receptor.
    J Gen Virol. 1987 Mar;68 ( Pt 3):793-803 PMID: 3029306
  32. Human cytomegalovirus encodes a glycoprotein homologous to MHC class-I antigens.
    Nature. 1988 Jan 21;331(6153):269-72 PMID: 2827039
  33. Virus receptors on lymphoid cells.
    Methods Enzymol. 1987;150:548-58 PMID: 2828828
  34. HIV infection is blocked in vitro by recombinant soluble CD4.
    Nature. 1988 Jan 7;331(6151):76-8 PMID: 2829022
  35. A soluble CD4 protein selectively inhibits HIV replication and syncytium formation.
    Nature. 1988 Jan 7;331(6151):78-81 PMID: 2829023
  36. A soluble form of CD4 (T4) protein inhibits AIDS virus infection.
    Nature. 1988 Jan 7;331(6151):82-4 PMID: 3257544
  37. Soluble CD4 molecules neutralize human immunodeficiency virus type 1.
    Nature. 1988 Jan 7;331(6151):84-6 PMID: 2829024
  38. Model for studying virus attachment: identification and quantitation of Epstein-Barr virus-binding cells by using biotinylated virus in flow cytometry.
    J Virol. 1988 Jul;62(7):2453-63 PMID: 2836625
  39. Structure of the influenza virus haemagglutinin complexed with its receptor, sialic acid.
    Nature. 1988 Jun 2;333(6172):426-31 PMID: 3374584
  40. Location and chemical synthesis of a binding site for HIV-1 on the CD4 protein.
    Science. 1988 Jun 3;240(4857):1335-9 PMID: 2453925
  41. Synthetic CD4 peptide derivatives that inhibit HIV infection and cytopathicity.
    Science. 1988 Aug 5;241(4866):712-6 PMID: 2969619
  42. Effect of recombinant soluble CD4 in rhesus monkeys infected with simian immunodeficiency virus of macaques.
    Nature. 1989 Jan 19;337(6204):267-70 PMID: 2536140
  43. Identification of an epitope in the major envelope protein of Epstein-Barr virus that mediates viral binding to the B lymphocyte EBV receptor (CR2).
    Cell. 1989 Feb 10;56(3):369-77 PMID: 2464439
  44. Characterization of a common high-affinity receptor for reovirus serotypes 1 and 3 on endothelial cells.
    J Virol. 1989 Mar;63(3):1318-25 PMID: 2915382
  45. The major human rhinovirus receptor is ICAM-1.
    Cell. 1989 Mar 10;56(5):839-47 PMID: 2538243
  46. A cell adhesion molecule, ICAM-1, is the major surface receptor for rhinoviruses.
    Cell. 1989 Mar 10;56(5):849-53 PMID: 2538244
  47. Human cytomegalovirus binding to fibroblasts is receptor mediated.
    J Virol. 1989 Sep;63(9):3991-8 PMID: 2548011
  48. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):763-7 PMID: 6096719
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.6.2484-2490.1990
Published
1990-06-00
Pages
2484-90
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249423
Subset
IM
Grants
NIAID NIH HHS · AI17354 · United States
NIAID NIH HHS · AI25016 · United States
NCI NIH HHS · CA14692 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.6.2484-2490.1990 PMC Full Text View journal details
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