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PMID: 2557618 Published · ppublish English Journal Article

Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 10100-3

Keay S, Merigan TC, Rasmussen L

Abstract

Cell surface receptors for the 86-kDa glycoprotein (gp86) of human cytomegalovirus (HCMV) were identified by using two monoclonal anti-idiotype antibodies that bear the internal image of gp86. These antibodies bound to cells permissive for HCMV infection by both ELISA and immunofluorescence assay and inhibited HCMV plaque formation in human embryonic lung (HEL) cells. Immunoblot analysis showed specific binding of both internal image anti-idiotype antibodies as well as gp86 to an HEL cell membrane protein with an approximate molecular mass of 92.5 kDa. In addition, immunoprecipitation of radiolabeled membrane and cell surface proteins from human foreskin tissue, human foreskin fibroblasts, or HEL cells showed specific binding of anti-idiotype antibody predominantly to the 92.5-kDa protein.

MeSH Terms
Antibodies, Monoclonal Antigens, Viral/isolation & purification Cell Line Cell Membrane/metabolism Cytomegalovirus/growth & development,immunology,physiology Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Glycoproteins/isolation & purification,metabolism Humans Immunoblotting Immunoglobulin Idiotypes/immunology Molecular Weight Receptors, Virus/isolation & purification,metabolism Viral Plaque Assay
Chemicals
Antibodies, Monoclonal Antigens, Viral Glycoproteins Immunoglobulin Idiotypes Receptors, Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Keay S
Department of Medicine, Stanford University School of Medicine, CA 94305.
Merigan T C
Rasmussen L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.24.10100
Published
1989-12-00
Pages
10100-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298652
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.24.10100 PMC Full Text View journal details
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