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PMID: 1903428 Published · ppublish English Journal Article

The gene coding for a major tumor rejection antigen of tumor P815 is identical to the normal gene of syngeneic DBA/2 mice.

The Journal of experimental medicine ·Vol. 173 ·No. 6 ·1991-06-01 ·Pages 1373-84

Van den Eynde B, Lethé B, Van Pel A, De Plaen E, Boon T

Abstract

We showed previously that mouse mastocytoma P815 expresses several distinct antigens that are recognized by cytolytic T lymphocytes (CTL) of syngeneic DBA/2 mice. Antigens P815A and P815B are usually lost jointly and are targets for immune rejection responses in vivo. We used a cosmid library and a CTL stimulation assay to obtain transfectants expressing tumor rejection antigen P815A. From these transfectants we retrieved gene P1A which transferred the expression of both P815A and B. This gene is unrelated to three previously isolated genes coding for tum-antigens. It encodes a putative protein of 224 amino acids which contains two highly acidic domains showing homology with similar regions of nuclear proteins. The P1A gene expressed by tumor P815 is completely identical to the gene present in normal DBA/2 cells. Expression of the gene was tested by Northern blots. Cells from liver, spleen, and a number of mast cell lines were negative, but mast cell line L138.8A produced a high level of P1A message and was lysed by CTL directed against antigens P815A and B. We conclude that major tumor rejection antigens of P815 are encoded by a gene showing little or no expression in most normal cells of adult mice.

Related Genes
P1A
MeSH Terms
Amino Acid Sequence Animals Antigen-Presenting Cells/immunology Antigens, Neoplasm/genetics Base Sequence Blotting, Northern Cloning, Molecular Cytotoxicity, Immunologic Gene Expression Genes H-2 Antigens/immunology Immunity, Cellular Mast-Cell Sarcoma/immunology Mice Mice, Inbred Strains Molecular Sequence Data Restriction Mapping Tumor Cells, Cultured/immunology
Chemicals
Antigens, Neoplasm H-2 Antigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Van den Eynde B
Ludwig Institute for Cancer Research, Brussels, Belgium.
Lethé B
Van Pel A
De Plaen E
Boon T
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45 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.173.6.1373
Published
1991-06-01
Pages
1373-84
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190831
Subset
IM
Databases
GENBANK
M36386, M36387, X58203, X58204, X58205, X58206, X58207, X58208, X58209, X58210
External Links
PubMed source DOI: 10.1084/jem.173.6.1373 PMC Full Text View journal details
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