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PMID: 19034478 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Preservation of photoreceptors in dystrophic RCS rats following allo- and xenotransplantation of IPE cells.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie ·Vol. 247 ·No. 3 ·2009-03-00 ·Pages 363-9

Thumann G, Salz AK, Walter P, Johnen S

Abstract

To examine whether iris pigment epithelial (IPE) cells transplanted into the subretinal space of Royal College of Surgeons (RCS) rats have the ability to rescue photoreceptors. Rat IPE (rIPE) or human IPE (hIPE) cells were transplanted subretinally in 23-day-old RCS rats. Sham injection and transplantation of ARPE-19 cells served as controls. After 12 weeks, eyes were evaluated for photoreceptor survival by morphometric analysis and electron microscopy. Morphometric analysis showed photoreceptor rescue in all transplanted and sham-injected animals (number of photoreceptors/300 microm retina+/-sd: rIPE 41.67 +/- 28; hIPE 29.50 +/- 16; ARPE-19 36.12 +/- 21; sham 16.56 +/- 6) compared to age-matched, control rats (number of photoreceptors/300 microm retina+/-sd: 9.71 +/- 4). Photoreceptor rescue was prominent in IPE cell-transplanted rats and was significantly greater than sham-injected eyes (p = 0.02 for rIPE and p = 0.04 for hIPE). Since IPE cells transplanted into the subretinal space have the ability to rescue photoreceptors from degeneration in the RCS rat without any harmful effects, IPE cells may represent an ideal cell to genetically modify and thus carry essential genetic information for the repair of defects in the subretinal space.

MeSH Terms
Animals Cell Survival/physiology Cell Transplantation/methods Cells, Cultured Graft Survival/physiology Humans Iris/cytology Microscopy, Electron Photoreceptor Cells, Vertebrate/physiology,ultrastructure Pigment Epithelium of Eye/physiology,transplantation,ultrastructure Rats Rats, Long-Evans Rats, Mutant Strains Retinal Degeneration/pathology,surgery Transplantation, Heterologous Transplantation, Homologous
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thumann Gabriele
IZKF Biomat, RWTH University of Aachen, Pauwelsstrasse 30, 52074, Aachen, Germany. [email protected]
Salz Anna Katharina
Walter Peter
Johnen Sandra
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Article Info
Journal
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
Abbr.
Graefes Arch Clin Exp Ophthalmol
ISSN
1435-702X
DOI
10.1007/s00417-008-0998-x
Email
[email protected]
Published
2009-03-00
Epub
2008-00-26
Pages
363-9
Language
English
Region
Germany
NLM ID
8205248
Subset
IM
External Links
PubMed source DOI: 10.1007/s00417-008-0998-x View journal details
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