Primary structure, expression in Escherichia coli, and properties of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Bacillus megaterium.
A Bacillus megaterium DNA fragment encoding S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase (SUMT) activity was subcloned and sequenced. The encoded polypeptide showed more than 43.5% strict homology to Pseudomonas denitrificans SUMT (F. Blanche, L. Debussche, D. Thibaut, J. Crouzet, and B. Cameron, J. Bacteriol. 171:4222-4231, 1989). The B. megaterium polypeptide was overexpressed in Escherichia coli, partially purified, and shown to exhibit, like P. denitrificans SUMT, substrate inhibition at uroporphyrinogen III concentrations above 0.5 microM, suggesting a common regulation for aerobic cobalamin-producing organisms.
17 references, click to expand
-
Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase.J Bacteriol. 1990 Oct;172(10):5968-79 PMID: 2211520
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
-
Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.Gene. 1988 Oct 15;70(1):191-7 PMID: 2853689
-
Cloning of multiple genes involved with cobalamin (Vitamin B12) biosynthesis in Bacillus megaterium.J Bacteriol. 1986 Aug;167(2):623-30 PMID: 3015883
-
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.Gene. 1985;33(1):103-19 PMID: 2985470
-
Purification and characterization of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans.J Bacteriol. 1989 Aug;171(8):4222-31 PMID: 2546914
-
The biosynthesis of porphyrins, chlorophylls, and vitamin B12.Nat Prod Rep. 1989 Apr;6(2):171-203 PMID: 2664584
-
Chemical synthesis of a gene coding for human angiogenin, its expression in Escherichia coli and conversion of the product into its active form.Gene. 1987;56(1):61-70 PMID: 3315860
-
Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.Eur J Biochem. 1990 Jul 31;191(2):315-23 PMID: 2200672
-
The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase.Biochem J. 1990 Feb 1;265(3):725-9 PMID: 2407234
-
Uroporphyrinogen III, an intermediate in the biosynthesis of the nickel-containing factor F430 in Methanobacterium thermoautotrophicum.Eur J Biochem. 1983 Sep 1;135(1):109-12 PMID: 6884352
-
Translational initiation in prokaryotes.Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
-
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.Gene. 1984 Jun;28(3):351-9 PMID: 6235151
-
Use of statistical criteria for screening potential homologies in nucleic acid sequences.Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):203-13 PMID: 6694901
-
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.Gene. 1977;2(2):95-113 PMID: 344137
-
Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans.J Bacteriol. 1990 Nov;172(11):6239-44 PMID: 2172209
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