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PMID: 19079549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus.

PloS one ·Vol. 3 ·No. 12 ·2008-00-00 ·Pages e3921

Kaito C, Omae Y, Matsumoto Y, Nagata M, Yamaguchi H, Aoto T, Ito T, Hiramatsu K, Sekimizu K

Abstract

Staphylococcus aureus colonies can spread on soft agar plates. We compared colony spreading of clinically isolated methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). All MSSA strains showed colony spreading, but most MRSA strains (73%) carrying SCCmec type-II showed little colony spreading. Deletion of the entire SCCmec type-II region from these MRSA strains restored colony spreading. Introduction of a novel gene, fudoh, carried by SCCmec type-II into Newman strain suppressed colony spreading. MRSA strains with high spreading ability (27%) had no fudoh or a point-mutated fudoh that did not suppress colony spreading. The fudoh-transformed Newman strain had decreased exotoxin production and attenuated virulence in mice. Most community-acquired MRSA strains carried SCCmec type-IV, which does not include fudoh, and showed high colony spreading ability. These findings suggest that fudoh in the SCCmec type-II region suppresses colony spreading and exotoxin production, and is involved in S. aureus pathogenesis.

MeSH Terms
Animals Colony Count, Microbial Exotoxins/metabolism Genes, Bacterial Methicillin Resistance/genetics Mice Residence Characteristics Sequence Deletion Staphylococcus aureus/genetics,isolation & purification,pathogenicity Transformation, Bacterial Virulence
Chemicals
Exotoxins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kaito Chikara
Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.
Omae Yosuke
Matsumoto Yasuhiko
Nagata Makiko
Yamaguchi Hiroki
Aoto Taiji
Ito Teruyo
Hiramatsu Keiichi
Sekimizu Kazuhisa
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0003921
Published
2008-00-00
Epub
2008-00-11
Pages
e3921
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2593785
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0003921 PMC Full Text View journal details
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