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PMID: 19081079 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Hoxb5b acts downstream of retinoic acid signaling in the forelimb field to restrict heart field potential in zebrafish.

Developmental cell ·Vol. 15 ·No. 6 ·2008-12-00 ·Pages 923-34

Waxman JS, Keegan BR, Roberts RW, Poss KD, Yelon D

Abstract

How adjacent organ fields communicate during development is not understood. Here, we identify a mechanism in which signaling within the forelimb field restricts the potential of the neighboring heart field. In zebrafish embryos deficient in retinoic acid (RA) signaling, the pectoral fins (forelimbs) are lost while both chambers of the heart are enlarged. We provide evidence that both of these phenotypes are due to RA signaling acting directly within the forelimb field. hoxb5b, an RA-responsive gene expressed within the forelimb field, is required to restrict the number of atrial cells arising from the adjacent heart field, although its function is dispensable for forelimb formation. Together, these data indicate nonautonomous influences downstream of RA signaling that act to limit individual chamber size. Therefore, our results offer new perspectives on the mechanisms regulating organ size and the possible causes of congenital syndromes affecting both the heart and forelimb.

MeSH Terms
Animals Apoptosis Cell Lineage Forelimb/embryology Gene Expression Regulation, Developmental Heart/embryology Heart Atria/embryology Heart Ventricles/embryology Homeodomain Proteins/biosynthesis,physiology Models, Biological Phenotype Signal Transduction Time Factors Tretinoin/metabolism Zebrafish Zebrafish Proteins/biosynthesis,metabolism,physiology
Chemicals
Homeodomain Proteins Zebrafish Proteins hoxb5b protein, zebrafish Tretinoin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Waxman Joshua S
Department of Cell Biology, Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA.
Keegan Brian R
Roberts Richard W
Poss Kenneth D
Yelon Deborah
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1878-1551
DOI
10.1016/j.devcel.2008.09.009
Published
2008-12-00
Pages
923-34
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC2752051
Subset
IM
Grants
NHLBI NIH HHS · R01 HL069594-07 · United States
NHLBI NIH HHS · R01 HL069594-04 · United States
NICHD NIH HHS · T32 HD007520 · United States
NHLBI NIH HHS · F32 HL083591 · United States
NHLBI NIH HHS · R01 HL069594-06 · United States
NHLBI NIH HHS · R01 HL069594-03 · United States
NHLBI NIH HHS · F32 HL083591-02 · United States
NHLBI NIH HHS · R01 HL081674-03 · United States
NHLBI NIH HHS · K99 HL091126-01 · United States
NHLBI NIH HHS · R01 HL069594-02 · United States
NHLBI NIH HHS · F32 HL083591-01 · United States
NHLBI NIH HHS · K99 HL091126 · United States
NHLBI NIH HHS · R01 HL069594-01 · United States
NHLBI NIH HHS · R01 HL069594-05A1 · United States
NHLBI NIH HHS · R01 HL081674 · United States
NHLBI NIH HHS · R01 HL069594 · United States
External Links
PubMed source DOI: 10.1016/j.devcel.2008.09.009 PMC Full Text View journal details
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