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PMID: 1908829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mechanisms involved in mycobacterial growth inhibition by gamma interferon-activated bone marrow macrophages: role of reactive nitrogen intermediates.

Infection and immunity ·Vol. 59 ·No. 9 ·1991-09-00 ·Pages 3213-8

Flesch IE, Kaufmann SH

Abstract

Murine bone marrow-derived macrophages are able to inhibit the growth of Mycobacterium bovis after stimulation with recombinant gamma interferon. This antimycobacterial activity was inhibited by NG-monomethyl-L-arginine, a specific inhibitor of nitrite and nitrate synthesis from L-arginine. Furthermore, there was a complete lack of mycobacterial growth inhibition in a medium deficient in L-arginine. Nitrite is generated by gamma interferon-activated bone marrow-derived macrophages after infection with M. bovis, and a correlation between mycobacterial growth inhibition and nitrite production was observed. These results indicate that reactive nitrogen intermediates derived from L-arginine are crucially involved in macrophage antimycobacterial activity.

MeSH Terms
Animals Arginine/analogs & derivatives,metabolism,pharmacology Bone Marrow/immunology Bone Marrow Cells Cells, Cultured Female Hydrogen-Ion Concentration Interferon-gamma/pharmacology Lymphocyte Activation Macrophages/immunology,metabolism Male Mice Mice, Inbred C57BL Mycobacterium bovis/growth & development Nitrogen Oxides/antagonists & inhibitors,metabolism Recombinant Proteins omega-N-Methylarginine
Chemicals
Nitrogen Oxides Recombinant Proteins omega-N-Methylarginine Interferon-gamma Arginine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Flesch I E
Department of Immunology, University of Ulm, Federal Republic of Germany.
Kaufmann S H
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.9.3213-3218.1991
Published
1991-09-00
Pages
3213-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258155
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.59.9.3213-3218.1991 PMC Full Text View journal details
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