Home LiteratureArticle Details
PMID: 19088331 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

ASYMMETRIC LEAVES2-LIKE19/LATERAL ORGAN BOUNDARIES DOMAIN30 and ASL20/LBD18 regulate tracheary element differentiation in Arabidopsis.

The Plant cell ·Vol. 20 ·No. 12 ·2008-12-00 ·Pages 3359-73

Soyano T, Thitamadee S, Machida Y, Chua NH

Abstract

ASYMMETRIC LEAVES2 (AS2)/LATERAL ORGAN BOUNDARIES DOMAIN (LBD) family proteins are plant-specific nuclear proteins, and genes encoding several family members have been implicated in plant development. We investigated the function of two members of the Arabidopsis thaliana AS2/LBD family, AS2-LIKE19 (ASL19)/LBD30 and ASL20/LBD18, which encode homologous proteins. Both ASL19 and ASL20 were expressed in immature tracheary elements (TEs), and the expression was dependent on VASCULAR-RELATED NAC-DOMAIN PROTEIN6 (VND6) and VND7, which are transcription factors required for TE differentiation. Overexpression of ASL19 and ASL20 induced transdifferentiation of cells from nonvascular tissues into TE-like cells, similar to those induced upon VND6/7 overexpression. By contrast, aberrant TEs were formed when a cDNA encoding a fusion protein of ASL20 with an artificial repressor domain (ASL20-SRDX) was expressed from its native promoter. These results provide evidence that ASL proteins positively regulate TE differentiation. In transgenic plants overexpressing both ASL19 and ASL20, the xylem-deficient phenotype caused by the expression of dominant-negative versions of VND6/7 proteins was not rescued. However, ectopic expression of VND7 was detected in plants overexpressing ASL20. Moreover, VND genes and their downstream targets were downregulated in ASL20-SRDX plants. Therefore, ASL20 appears to be involved in a positive feedback loop for VND7 expression that regulates TE differentiation-related genes.

MeSH Terms
Arabidopsis/cytology,genetics,metabolism Arabidopsis Proteins/genetics,metabolism,physiology Cell Differentiation/genetics,physiology Microscopy Plants, Genetically Modified/cytology,genetics,metabolism Polymerase Chain Reaction Recombinant Fusion Proteins/genetics,metabolism,physiology Xylem/cytology,genetics,metabolism
Chemicals
Arabidopsis Proteins Recombinant Fusion Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Soyano Takashi
Laboratory of Plant Molecular Biology, The Rockefeller University, New York, New York 10065, USA.
Thitamadee Siripong
Machida Yasunori
Chua Nam-Hai
References (46)
46 references, click to expand
  1. Control of cellulose synthase complex localization in developing xylem.
    Plant Cell. 2003 Aug;15(8):1740-8 PMID: 12897249
  2. ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis.
    Plant Cell. 2007 Jan;19(1):118-30 PMID: 17259263
  3. ARL1, a LOB-domain protein required for adventitious root formation in rice.
    Plant J. 2005 Jul;43(1):47-56 PMID: 15960615
  4. Regulation of secondary cell wall development by cortical microtubules during tracheary element differentiation in Arabidopsis cell suspensions.
    Plant Physiol. 2005 Mar;137(3):1027-36 PMID: 15709154
  5. The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis.
    Plant Physiol. 1999 Mar;119(3):897-907 PMID: 10069828
  6. The indeterminate gametophyte1 gene of maize encodes a LOB domain protein required for embryo Sac and leaf development.
    Plant Cell. 2007 Jan;19(1):46-62 PMID: 17209126
  7. A proteoglycan mediates inductive interaction during plant vascular development.
    Nature. 2004 Jun 24;429(6994):873-8 PMID: 15215864
  8. ASYMMETRIC LEAVES2-LIKE1 gene, a member of the AS2/LOB family, controls proximal-distal patterning in Arabidopsis petals.
    Plant Mol Biol. 2005 Mar;57(4):559-75 PMID: 15821980
  9. GATEWAY vectors for Agrobacterium-mediated plant transformation.
    Trends Plant Sci. 2002 May;7(5):193-5 PMID: 11992820
  10. The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves.
    Development. 2001 May;128(10):1771-83 PMID: 11311158
  11. The lateral organ boundaries gene defines a novel, plant-specific gene family.
    Plant Physiol. 2002 Jun;129(2):747-61 PMID: 12068116
  12. The irregular xylem3 locus of Arabidopsis encodes a cellulose synthase required for secondary cell wall synthesis.
    Plant Cell. 1999 May;11(5):769-80 PMID: 10330464
  13. Crown rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin signaling.
    Plant Cell. 2005 May;17(5):1387-96 PMID: 15829602
  14. The Arabidopsis xylem peptidase XCP1 is a tracheary element vacuolar protein that may be a papain ortholog.
    Plant Physiol. 2002 Jan;128(1):84-94 PMID: 11788755
  15. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
    Plant J. 1998 Dec;16(6):735-43 PMID: 10069079
  16. Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis.
    Cell. 1996 Apr 19;85(2):171-82 PMID: 8612270
  17. BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis.
    Development. 2004 Nov;131(21):5341-51 PMID: 15486337
  18. Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19.
    Plant Cell. 2005 Feb;17(2):444-63 PMID: 15659631
  19. Transcription switches for protoxylem and metaxylem vessel formation.
    Genes Dev. 2005 Aug 15;19(16):1855-60 PMID: 16103214
  20. The role of MAP65-1 in microtubule bundling during Zinnia tracheary element formation.
    J Cell Sci. 2006 Feb 15;119(Pt 4):753-8 PMID: 16449317
  21. Cytokinin signaling and its inhibitor AHP6 regulate cell fate during vascular development.
    Science. 2006 Jan 6;311(5757):94-8 PMID: 16400151
  22. Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics.
    Plant Cell. 2005 Aug;17(8):2281-95 PMID: 15980264
  23. The expression of the Athb-8 homeobox gene is restricted to provascular cells in Arabidopsis thaliana.
    Development. 1995 Dec;121(12):4171-82 PMID: 8575317
  24. ramosa2 encodes a LATERAL ORGAN BOUNDARY domain protein that determines the fate of stem cells in branch meristems of maize.
    Plant Cell. 2006 Mar;18(3):574-85 PMID: 16399802
  25. The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper.
    Plant Cell Physiol. 2002 May;43(5):467-78 PMID: 12040093
  26. Dodeca-CLE peptides as suppressors of plant stem cell differentiation.
    Science. 2006 Aug 11;313(5788):842-5 PMID: 16902140
  27. Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis.
    Plant Cell. 2008 Jan;20(1):48-58 PMID: 18203921
  28. Signals that control plant vascular cell differentiation.
    Nat Rev Mol Cell Biol. 2004 May;5(5):379-91 PMID: 15122351
  29. Novel as1 and as2 defects in leaf adaxial-abaxial polarity reveal the requirement for ASYMMETRIC LEAVES1 and 2 and ERECTA functions in specifying leaf adaxial identity.
    Development. 2003 Sep;130(17):4097-107 PMID: 12874130
  30. Vascular-related NAC-DOMAIN7 is involved in the differentiation of all types of xylem vessels in Arabidopsis roots and shoots.
    Plant J. 2008 Aug;55(4):652-64 PMID: 18445131
  31. Mechanisms that control knox gene expression in the Arabidopsis shoot.
    Development. 2000 Dec;127(24):5523-32 PMID: 11076771
  32. The ATE genes are responsible for repression of transdifferentiation into xylem cells in Arabidopsis.
    Plant Physiol. 2005 Jan;137(1):141-8 PMID: 15618413
  33. Mechanism for formation of the secondary wall thickening in tracheary elements: Microtubules and microfibrils of tracheary elements of Pisum sativum L. and Commelina communis L. and the effects of amiprophosmethyl.
    Planta. 1991 Sep;185(2):190-200 PMID: 24186341
  34. Spatial and temporal tracing of vessel differentiation in young Arabidopsis seedlings by the expression of an immature tracheary element-specific promoter.
    Plant Cell Physiol. 2004 Oct;45(10):1529-36 PMID: 15564536
  35. Technical advance: An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants.
    Plant J. 2000 Oct;24(2):265-73 PMID: 11069700
  36. Activation tagging, a novel tool to dissect the functions of a gene family.
    Plant J. 2003 Jun;34(5):741-50 PMID: 12787254
  37. Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis.
    Plant J. 2003 Jun;34(5):733-9 PMID: 12787253
  38. Stage-specific markers define early steps of procambium development in Arabidopsis leaves and correlate termination of vein formation with mesophyll differentiation.
    Development. 2004 Jul;131(14):3445-55 PMID: 15226260
  39. A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants.
    Plant Cell. 1994 Dec;6(12):1859-76 PMID: 7866029
  40. LATERAL ORGAN BOUNDARIES defines a new family of DNA-binding transcription factors and can interact with specific bHLH proteins.
    Nucleic Acids Res. 2007;35(19):6663-71 PMID: 17913740
  41. Arabidopsis JAGGED LATERAL ORGANS is expressed in boundaries and coordinates KNOX and PIN activity.
    Plant Cell. 2007 Jun;19(6):1795-808 PMID: 17557810
  42. A link between cytokinin and ASL9 (ASYMMETRIC LEAVES 2 LIKE 9) that belongs to the AS2/LOB (LATERAL ORGAN BOUNDARIES) family genes in Arabidopsis thaliana.
    Biosci Biotechnol Biochem. 2007 May;71(5):1269-78 PMID: 17485849
  43. Brassinosteroids induce entry into the final stage of tracheary element differentiation in cultured Zinnia cells.
    Plant Cell Physiol. 1997 Aug;38(8):980-3 PMID: 9440936
  44. The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning.
    Plant Cell. 2003 Oct;15(10):2241-52 PMID: 14508003
  45. Tracheary element differentiation.
    Annu Rev Plant Biol. 2007;58:407-33 PMID: 17472568
  46. The maize (Zea mays L.) RTCS gene encodes a LOB domain protein that is a key regulator of embryonic seminal and post-embryonic shoot-borne root initiation.
    Plant J. 2007 May;50(4):649-59 PMID: 17425722
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.108.061796
Published
2008-12-00
Epub
2008-00-16
Pages
3359-73
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2630433
Subset
IM
Grants
NIGMS NIH HHS · R01 GM044640 · United States
NIGMS NIH HHS · R56 GM044640 · United States
NIGMS NIH HHS · GM-44640 · United States
External Links
PubMed source DOI: 10.1105/tpc.108.061796 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2