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PMID: 1909344 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Enzyme-linked immunosorbent assay using monoclonal antibodies for identification of mycobacteria from early cultures.

Journal of clinical microbiology ·Vol. 29 ·No. 7 ·1991-07-00 ·Pages 1372-5

Verstijnen CP, Ly HM, Polman K, Richter C, Smits SP, Maselle SY, Peerbooms P, Rienthong D, Montreewasuwat N, Koanjanart S

Abstract

A simple enzyme-linked immunosorbent assay (ELISA) for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii has been developed (R. Schöningh, C. P. H. J. Verstijnen, S. Kuijper, and A. H. J. Kolk. J. Clin. Microbiol. 28:708-713, 1990). The test for the routine identification of cultured mycobacteria was introduced in five clinical laboratories located in Tanzania, Thailand, Vietnam, and The Netherlands. The ELISA can be conducted without an ELISA reader since the test can be read visually. The results of identification of 255 strains of the M. tuberculosis complex by microbiological means and by ELISA were compared; the specificity and the sensitivity were 100%. For the M. avium complex, the specificity was 100% and the sensitivity was 64%. All 26 M. kansasii strains tested could be identified as M. kansasii. The ELISA described here proved to be useful in both well- and modestly equipped laboratories and may replace the microbiological method of identification of M. tuberculosis and M. kansasii.

MeSH Terms
Antibodies, Monoclonal Enzyme-Linked Immunosorbent Assay/methods Evaluation Studies as Topic Humans Mycobacterium/classification,immunology,isolation & purification Mycobacterium Infections/diagnosis Mycobacterium avium Complex/isolation & purification Mycobacterium tuberculosis/isolation & purification Nontuberculous Mycobacteria/isolation & purification Sensitivity and Specificity
Chemicals
Antibodies, Monoclonal
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Verstijnen C P
Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
Ly H M
Polman K
Richter C
Smits S P
Maselle S Y
Peerbooms P
Rienthong D
Montreewasuwat N
Koanjanart S
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.29.7.1372-1375.1991
Published
1991-07-00
Pages
1372-5
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC270120
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.29.7.1372-1375.1991 PMC Full Text View journal details
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