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PMID: 1915306 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Substrate specificity of the dsRNA unwinding/modifying activity.

The EMBO journal ·Vol. 10 ·No. 11 ·1991-11-00 ·Pages 3523-32

Nishikura K, Yoo C, Kim U, Murray JM, Estes PA, Cash FE, Liebhaber SA

Abstract

Double-stranded RNA (dsRNA) unwinding/modifying activity, which is present in a wide range of eukaryotic cells, has been previously shown to convert up to 50% of adenosine residues to inosines within intermolecular dsRNA. In the present study, we report that this activity also modifies, though slightly less efficiently, intramolecular double-stranded regions of synthetic RNAs. Our results widen the range of the possible biological substrates for the activity since many stem and loop type RNA secondary structures (intramolecular dsRNA), present in eukaryotic as well as viral transcripts, can potentially serve as substrates. In addition, we have found that the dsRNA unwinding/modifying activity requires a double-stranded region of at least 15-20 base pairs (bp) for substrate recognition. Furthermore, modification efficiency was found to be critically dependent on the length of the double-stranded region; as the size decreased below 100 bp, it dropped precipitously. Our results suggest that efficient modification may occur only with relatively long (greater than 100 bp) dsRNA, perhaps because multiple copies of the enzyme must be bound.

MeSH Terms
Base Sequence Binding, Competitive DNA/genetics Globins/genetics Growth Hormone/genetics HeLa Cells Humans Molecular Sequence Data Nucleic Acid Conformation Plasmids RNA, Double-Stranded/genetics,metabolism
Chemicals
RNA, Double-Stranded Growth Hormone Globins DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nishikura K
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Yoo C
Kim U
Murray J M
Estes P A
Cash F E
Liebhaber S A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-11-00
Pages
3523-32
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453081
Subset
IM
Grants
NCI NIH HHS · R01 CA46676 · United States
NIGMS NIH HHS · R01 GM40536 · United States
External Links
PubMed source PMC Full Text View journal details
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