Home LiteratureArticle Details
PMID: 1920385 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assembly and sealing of tight junctions: possible participation of G-proteins, phospholipase C, protein kinase C and calmodulin.

The Journal of membrane biology ·Vol. 122 ·No. 3 ·1991-06-00 ·Pages 193-202

Balda MS, González-Mariscal L, Contreras RG, Macias-Silva M, Torres-Marquez ME, García-Sáinz JA, Cereijido M

Abstract

The making and sealing of a tight junction (TJ) requires cell-cell contacts and Ca2+, and can be gauged through the development of transepithelial electrical resistance (TER) and the accumulation of ZO-1 peptide at the cell borders. We observe that pertussis toxin increases TER, while AIF3 and carbamil choline (carbachol) inhibit it, and 5-guanylylimidodiphosphate (GTPTs) blocks the development of a cell border pattern of ZO-1, suggesting that G-proteins are involved. Phospholipase C (PLC) and protein kinase C (PKC) probably participate in these processes since (i) activation of PLC by thyrotropin-1 releasing hormone increases TER, and its inhibition by neomycin blocks the development of this resistance; (ii) 1,2-dioctanoylglycerol, an activator of PKC, stimulates TER development, while polymyxin B and 1-(5-isoquinoline sulfonyl)-2-methyl-piperazine dihydrochloride (H7), which inhibit this enzyme, abolish TER. Addition of 3-isobutyl-1-methyl-xanthine, dB-cAMP or forskolin do not enhance the value of TER, but have just the opposite effect. Trifluoperazine and calmidazoline inhibit TER development, suggesting that calmodulin (CaM) also plays a role in junction formation. These results indicate that junction formation may be controlled by a network of reactions where G-proteins, phospholipase C, adenylate cyclase, protein kinase C and CaM are involved.

MeSH Terms
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Adenylate Cyclase Toxin Animals Calcium/physiology Calmodulin/physiology Carbachol/pharmacology Cells, Cultured Electric Conductivity/drug effects,physiology Epithelial Cells Epithelium/metabolism,physiology GTP-Binding Proteins/physiology Intercellular Junctions/metabolism,physiology Isoquinolines/pharmacology Kidney/cytology,metabolism,physiology Membrane Potentials/drug effects,physiology Neomycin/pharmacology Pertussis Toxin Piperazines/pharmacology Polymyxin B/pharmacology Protein Kinase C/antagonists & inhibitors,physiology Trifluoperazine/pharmacology Type C Phospholipases/physiology Virulence Factors, Bordetella/pharmacology
Chemicals
Adenylate Cyclase Toxin Calmodulin Isoquinolines Piperazines Virulence Factors, Bordetella Trifluoperazine 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Carbachol Pertussis Toxin Protein Kinase C Type C Phospholipases GTP-Binding Proteins Neomycin Polymyxin B Calcium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Balda M S
Center for Research and Advanced Studies, Department of Physiology and Biophysics, D.F., Mexico.
González-Mariscal L
Contreras R G
Macias-Silva M
Torres-Marquez M E
García-Sáinz J A
Cereijido M
References (45)
45 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Hormonal regulation of hepatocyte tight junctional permeability.
    Am J Physiol. 1988 Oct;255(4 Pt 1):G454-61 PMID: 2845804
  3. Steps in the morphogenesis of a polarized epithelium. II. Disassembly and assembly of plasma membrane domains during reversal of epithelial cell polarity in multicellular epithelial (MDCK) cysts.
    J Cell Sci. 1990 Jan;95 ( Pt 1):153-65 PMID: 2351700
  4. Pertussis toxin. Affinity purification of a new ADP-ribosyltransferase.
    J Biol Chem. 1983 Dec 10;258(23):14647-51 PMID: 6315733
  5. Inhibition by phenothiazine antipsychotic drugs of calcium-dependent phosphorylation of cerebral cortex proteins regulated by phospholipid or calmodulin.
    Life Sci. 1981 Aug 17;29(7):725-33 PMID: 7278508
  6. Tight junction formation in cultured epithelial cells (MDCK).
    J Membr Biol. 1985;86(2):113-25 PMID: 4032460
  7. Characterization of ZO-1, a protein component of the tight junction from mouse liver and Madin-Darby canine kidney cells.
    J Cell Biol. 1988 Apr;106(4):1141-9 PMID: 2452168
  8. Angiotensin II and dopamine modulate both cAMP and inositol phosphate productions in anterior pituitary cells. Involvement in prolactin secretion.
    J Biol Chem. 1986 Mar 25;261(9):4071-5 PMID: 3005316
  9. Dissociation of Madin-Darby canine kidney epithelial cells by the monoclonal antibody anti-arc-1: mechanistic aspects and identification of the antigen as a component related to uvomorulin.
    J Cell Biol. 1985 Oct;101(4):1307-15 PMID: 2995405
  10. Intracellular activation of protein kinase C and regulation of the surface transferrin receptor by diacylglycerol is a spontaneously reversible process that is associated with rapid formation of phosphatidic acid.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1281-4 PMID: 3006042
  11. Thyrotropin-releasing hormone activates a Ca2+-dependent polyphosphoinositide phosphodiesterase in permeable GH3 cells. GTP gamma S potentiation by a cholera and pertussis toxin-insensitive mechanism.
    J Biol Chem. 1986 Feb 25;261(6):2918-27 PMID: 3005271
  12. Evidence implicating protein kinase C in exocytosis from electropermeabilized bovine chromaffin cells.
    J Membr Biol. 1988 Aug;104(1):21-34 PMID: 3184176
  13. Cytoplasmic regulation of tight-junction permeability: effect of plant cytokinins.
    Am J Physiol. 1980 Sep;239(3):C75-89 PMID: 7435552
  14. Fluoroaluminates activate transducin-GDP by mimicking the gamma-phosphate of GTP in its binding site.
    FEBS Lett. 1985 Oct 28;191(2):181-5 PMID: 3863758
  15. A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide.
    J Cell Biol. 1986 Feb;102(2):457-68 PMID: 3511070
  16. Role of calcium in tight junction formation between epithelial cells.
    Am J Physiol. 1990 Dec;259(6 Pt 1):C978-86 PMID: 2124417
  17. Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.
    J Cell Biol. 1986 Sep;103(3):755-66 PMID: 3528172
  18. The epithelial tight junction: structure, function and preliminary biochemical characterization.
    Mol Cell Biochem. 1988 Oct;83(2):129-45 PMID: 3059173
  19. Increases in guinea pig small intestinal transepithelial resistance induced by osmotic loads are accompanied by rapid alterations in absorptive-cell tight-junction structure.
    J Cell Biol. 1983 Jul;97(1):125-36 PMID: 6863387
  20. Phosphorylation of the tight-junction protein ZO-1 in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance.
    Biochem J. 1989 Oct 15;263(2):597-9 PMID: 2597123
  21. Cell-adhesion molecule uvomorulin is localized in the intermediate junctions of adult intestinal epithelial cells.
    J Cell Biol. 1985 Jan;100(1):327-32 PMID: 3880756
  22. Evidence that a guanine nucleotide-binding protein linked to a muscarinic receptor inhibits directly phospholipase C.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4889-93 PMID: 2162060
  23. Bradykinin-activated membrane-associated phospholipase C in Madin-Darby canine kidney cells.
    J Clin Invest. 1988 Jun;81(6):1896-902 PMID: 2838525
  24. NADP+ enhances cholera and pertussis toxin-catalyzed ADP-ribosylation of membrane proteins.
    J Cyclic Nucleotide Protein Phosphor Res. 1986;11(4):265-74 PMID: 3025276
  25. Receptors that inhibit phosphoinositide breakdown.
    Trends Pharmacol Sci. 1989 Mar;10(3):114-20 PMID: 2556822
  26. Activation of protein kinase C triggers premature compaction in the four-cell stage mouse embryo.
    Dev Biol. 1990 Mar;138(1):1-15 PMID: 2407575
  27. Structural basis for physiological regulation of paracellular pathways in intestinal epithelia.
    J Membr Biol. 1987;100(2):149-64 PMID: 3430571
  28. Teleost chloride cell. II. Autoradiographic localization of gill Na,K-ATPase in killifish Fundulus heteroclitus adapted to low and high salinity environments.
    J Cell Biol. 1976 Jul;70(1):157-77 PMID: 132451
  29. Inositol phosphates and cell signalling.
    Nature. 1989 Sep 21;341(6239):197-205 PMID: 2550825
  30. The role of the cell adhesion molecule uvomorulin in the formation and maintenance of the epithelial junctional complex.
    J Cell Biol. 1988 Oct;107(4):1575-87 PMID: 3049625
  31. Role of a guanine nucleotide-binding regulatory protein in the hydrolysis of hepatocyte phosphatidylinositol 4,5-bisphosphate by calcium-mobilizing hormones and the control of cell calcium. Studies utilizing aluminum fluoride.
    J Biol Chem. 1985 Nov 25;260(27):14477-83 PMID: 2997209
  32. Changes in tight junctions of thyroid epithelium with changes in thyroid activity.
    J Cell Biol. 1975 Sep;66(3):657-63 PMID: 1158975
  33. Collagen stimulates [3H]inositol trisphosphate formation in indomethacin-treated human platelets.
    Biochem J. 1985 Mar 15;226(3):831-7 PMID: 3872656
  34. Phorbol ester and neomycin dissociate bradykinin receptor-mediated arachidonic acid release and polyphosphoinositide hydrolysis in Madin-Darby canine kidney cells. Evidence that bradykinin mediates noninterdependent activation of phospholipases A2 and C.
    J Biol Chem. 1988 Oct 15;263(29):14640-7 PMID: 2902085
  35. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  36. Involvement of protein kinase C in translocation of desmoplakins from cytosol to plasma membrane during desmosome formation in human squamous cell carcinoma cells grown in low to normal calcium concentration.
    Exp Cell Res. 1989 Nov;185(1):176-90 PMID: 2509226
  37. Phospholipid-sensitive Ca2+-dependent protein kinase inhibition by R-24571, a calmodulin antagonist.
    Biochem Pharmacol. 1984 Jan 1;33(1):125-30 PMID: 6584106
  38. Molecular properties of the muscarinic acetylcholine receptor.
    Annu Rev Neurosci. 1987;10:195-236 PMID: 2436543
  39. Polarized monolayers formed by epithelial cells on a permeable and translucent support.
    J Cell Biol. 1978 Jun;77(3):853-80 PMID: 567227
  40. myo-Inositol 1,4,5-trisphosphate stimulates protein phosphorylation in saponin-permeabilized human platelets.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7431-5 PMID: 6438636
  41. The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species.
    EMBO J. 1989 Jun;8(6):1711-7 PMID: 2788574
  42. Phorbol ester, 1,2-diacylglycerol, and collagen induce inhibition of arachidonic acid incorporation into phospholipids in human platelets.
    J Biol Chem. 1989 Mar 5;264(7):3890-5 PMID: 2492991
  43. Experimental modulation of occluding junctions in a cultured transporting epithelium.
    J Cell Biol. 1980 Dec;87(3 Pt 1):736-45 PMID: 6780571
  44. [3H]GDP release from rat and hamster adipocyte membranes independently linked to receptors involved in activation or inhibition of adenylate cyclase. Differential susceptibility to two bacterial toxins.
    J Biol Chem. 1984 Jan 25;259(2):761-9 PMID: 6319385
  45. Bradykinin-evoked modulation of cytosolic Ca2+ concentrations in cultured renal epithelial (MDCK) cells.
    Biochem Biophys Res Commun. 1985 Jul 31;130(2):807-13 PMID: 3927911
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01871420
Published
1991-06-00
Pages
193-202
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01871420 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2