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PMID: 1922034 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats.

Molecular and cellular biology ·Vol. 11 ·No. 10 ·1991-10-00 ·Pages 5101-12

Flick JS, Johnston M

Abstract

Growth of the yeast Saccharomyces cerevisiae on glucose leads to repression of transcription of many genes required for alternative carbohydrate metabolism. The GRR1 gene appears to be of central importance to the glucose repression mechanism, because mutations in GRR1 result in a pleiotropic loss of glucose repression (R. Bailey and A. Woodword, Mol. Gen. Genet. 193:507-512, 1984). We have isolated the GRR1 gene and determined that null mutants are viable and display a number of growth defects in addition to the loss of glucose repression. Surprisingly, grr1 mutations convert SUC2, normally a glucose-repressed gene, into a glucose-induced gene. GRR1 encodes a protein of 1,151 amino acids that is expressed constitutively at low levels in yeast cells. GRR1 protein contains 12 tandem repeats of a sequence similar to leucine-rich motifs found in other proteins that may mediate protein-protein interactions. Indeed, cell fractionation studies are consistent with this view, suggesting that GRR1 protein is tightly associated with a particulate protein fraction in yeast extracts. The combined genetic and molecular data are consistent with the idea that GRR1 protein is a primary response element in the glucose repression pathway and is required for the generation or interpretation of the signal that induces glucose repression.

MeSH Terms
Amino Acid Sequence Base Sequence Carrier Proteins Cloning, Molecular DNA Mutational Analysis F-Box Proteins Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal/genetics Glucose/metabolism Immunoblotting Leucine/genetics Molecular Sequence Data Repetitive Sequences, Nucleic Acid/genetics Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligases
Chemicals
Carrier Proteins F-Box Proteins Fungal Proteins Saccharomyces cerevisiae Proteins GRR1 protein, S cerevisiae Ubiquitin-Protein Ligases Leucine Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Flick J S
Department of Genetics, Washington University Medical School, St. Louis, Missouri 63110.
Johnston M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.10.5101-5112.1991
Published
1991-10-00
Pages
5101-12
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361523
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032540 · United States
NIGMS NIH HHS · GM07067 · United States
NIGMS NIH HHS · GM08036 · United States
NIGMS NIH HHS · GM32540 · United States
Databases
GENBANK
M59247, M64827, M73024, M73025, M73026, M73027, S43616, S43620, S57499, X58858
External Links
PubMed source DOI: 10.1128/mcb.11.10.5101-5112.1991 PMC Full Text View journal details
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