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PMID: 1924351 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Copper resistance in Pseudomonas syringae mediated by periplasmic and outer membrane proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 20 ·1991-10-15 ·Pages 8915-9

Cha JS, Cooksey DA

Abstract

Copper-resistant strains of Pseudomonas syringae pathovar tomato accumulate copper and develop blue colonies on copper-containing media. Three of the protein products of the copper-resistance operon (cop) were characterized to provide an understanding of the copper-resistance mechanism and its relationship to copper accumulation. The Cop proteins, CopA (72 kDa), CopB (39 kDa), and CopC (12 kDa), were produced only under copper induction. CopA and CopC were periplasmic proteins and CopB was an outer membrane protein. Leader peptide sequences of CopA, CopB, and CopC were confirmed by amino-terminal peptide sequencing. CopA, CopB, and CopC were purified from strain PT23.2, and their copper contents were determined. One molecule of CopA bound 10.9 +/- 1.2 atoms of copper and one molecule of CopC bound 0.6 +/- 0.1 atom of copper. The Cop proteins apparently mediate sequestration of copper outside of the cytoplasm as a copper-resistance mechanism.

Related Genes
cop copA copB copC
MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/biosynthesis,genetics Bacterial Proteins/analysis,biosynthesis,genetics Cation Transport Proteins Copper/metabolism,pharmacology Drug Resistance, Microbial/genetics Immunoblotting Molecular Sequence Data Molecular Weight Operon Protein Sorting Signals/genetics Pseudomonas/drug effects,genetics Recombinant Fusion Proteins/analysis
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Cation Transport Proteins CopA protein, Bacteria CopC protein, Bacteria Protein Sorting Signals Recombinant Fusion Proteins Copper
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cha J S
Department of Plant Pathology, University of California, Riverside 92521-0122.
Cooksey D A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.20.8915
Published
1991-10-15
Pages
8915-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52621
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.20.8915 PMC Full Text View journal details
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