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PMID: 6315402 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Easy identification of cDNA clones.

The EMBO journal ·Vol. 2 ·No. 10 ·1983-00-00 ·Pages 1791-4

Rüther U, Müller-Hill B

Abstract

A set of six cloning vectors, pUR 278, 288, 289, 290, 291, 292 is presented. These vectors have the cloning sites, BamHI, SalI, PstI, XbaI and HindIII, in all frames at the 3' end of the lacZ gene. Insertion of cDNA in the proper cloning sites leads to a fusion protein of active beta-galactosidase and the peptide encoded by the cDNA. A simple immunoenzymatic assay can be used to identify clones in such a cDNA library.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Caseins/genetics Cloning, Molecular DNA/analysis DNA Restriction Enzymes Genetic Engineering/methods Genetic Vectors Muramidase/genetics Plasmids beta-Galactosidase/genetics
Chemicals
Caseins DNA DNA Restriction Enzymes Muramidase beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rüther U
Müller-Hill B
References (22)
22 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1791-4
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555360
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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