Home LiteratureArticle Details
PMID: 6170982 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of antigenic determinants of the hemagglutinin gene of a human influenza virus in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 9 ·1981-09-00 ·Pages 5376-80

Davis AR, Nayak DP, Ueda M, Hiti AL, Dowbenko D, Kleid DG

Abstract

Antigenic determinants of influenza virus hemagglutinin were expressed in Escherichia coli. DNA coding for presequences of hemagglutinin were removed and an ATG codon was placed before DNA coding for mature hemagglutinin. A number of expression plasmids were constructed in which various segments of this reconstructed hemagglutinin DNA were fused to DNA coding for bacterial beta-galactosidase. The fusion proteins exhibited specific binding to antiviral antibodies. This binding could be competitively inhibited by excess viral hemagglutinin, demonstrating that these fusion proteins contained antigenic determinants of hemagglutinin.

MeSH Terms
Cloning, Molecular/methods Epitopes Escherichia coli Genes, Synthetic Hemagglutinins, Viral/genetics Influenza A virus/genetics,immunology Plasmids
Chemicals
Epitopes Hemagglutinins, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Davis A R
Nayak D P
Ueda M
Hiti A L
Dowbenko D
Kleid D G
References (22)
22 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Complete sequence analysis shows that the hemagglutinins of the H0 and H2 subtypes of human influenza virus are closely related.
    Virology. 1981 May;111(1):113-24 PMID: 7233828
  3. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  4. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.
    Science. 1977 Dec 9;198(4321):1056-63 PMID: 412251
  5. Translation of the leader region of the Escherichia coli tryptophan operon.
    J Bacteriol. 1978 Mar;133(3):1457-66 PMID: 346574
  6. Antigenic determinants of influenza virus hemagglutinin. I. Cyanogen bromide peptides derived from A/MEMPHIS/72 hemagglutinin possess antigenic activity.
    Virology. 1978 Aug;89(1):199-205 PMID: 80057
  7. Evidence for a ninth influenza viral polypeptide.
    Virology. 1978 Nov;91(1):60-78 PMID: 726267
  8. Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril.
    Biochemistry. 1978 Oct 31;17(22):4807-17 PMID: 215191
  9. Structure of the haemagglutinin of influenza virus.
    Br Med Bull. 1979 Jan;35(1):57-63 PMID: 367488
  10. Expression in Escherichia coli of chemically synthesized genes for human insulin.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):106-10 PMID: 85300
  11. Antigenic determinants of influenza virus hemagglutinin. II. Antigenic reactivity of the isolated N-terminal cyanogen bromide peptide of A/Memphis/72 hemagglutinin heavy chain.
    Virology. 1979 Mar;93(2):458-65 PMID: 88123
  12. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.
    Nature. 1979 Oct 18;281(5732):544-8 PMID: 386136
  13. Influenza antigenic determinants are expressed from haemagglutinin genes cloned in Escherichia coli.
    Nature. 1980 Jan 10;283(5743):171-4 PMID: 6153123
  14. Selection of specific clones from colony banks by screening with radioactive antibody.
    Methods Enzymol. 1979;68:436-42 PMID: 396441
  15. Expression of cloned beta-endorphin gene sequences by Escherichia coli.
    Nature. 1980 Jun 12;285(5765):456-63 PMID: 6250034
  16. Human leukocyte interferon produced by E. coli is biologically active.
    Nature. 1980 Oct 2;287(5781):411-6 PMID: 6159538
  17. Synthesis of human fibroblast interferon by E. coli.
    Nucleic Acids Res. 1980 Sep 25;8(18):4057-74 PMID: 6159584
  18. Construction and characterization of a bacterial clone containing the hemagglutinin gene of the WSN strain (HON1) of influenza virus.
    Gene. 1980 Aug;10(3):205-18 PMID: 6254839
  19. Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.
    Nature. 1981 Jan 29;289(5796):366-73 PMID: 7464906
  20. Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation.
    Nature. 1981 Jan 29;289(5796):373-8 PMID: 6162101
  21. Production of biologically active N alpha-desacetylthymosin alpha 1 in Escherichia coli through expression of a chemically synthesized gene.
    Biochemistry. 1980 Dec 23;19(26):6096-104 PMID: 7008828
  22. Construction of plasmids carrying the cI gene of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 PMID: 1069307
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.9.5376
Published
1981-09-00
Pages
5376-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348748
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.9.5376 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2