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PMID: 19261596 Published · epublish English Journal Article

Odor coding by a Mammalian receptor repertoire.

Science signaling ·Vol. 2 ·No. 60 ·2009-03-03 ·Pages ra9

Saito H, Chi Q, Zhuang H, Matsunami H, Mainland JD

Abstract

Deciphering olfactory encoding requires a thorough description of the ligands that activate each odorant receptor (OR). In mammalian systems, however, ligands are known for fewer than 50 of more than 1400 human and mouse ORs, greatly limiting our understanding of olfactory coding. We performed high-throughput screening of 93 odorants against 464 ORs expressed in heterologous cells and identified agonists for 52 mouse and 10 human ORs. We used the resulting interaction profiles to develop a predictive model relating physicochemical odorant properties, OR sequences, and their interactions. Our results provide a basis for translating odorants into receptor neuron responses and for unraveling mammalian odor coding.

MeSH Terms
Algorithms Animals Flow Cytometry Humans Immunohistochemistry Luciferases Mice Models, Biological Odorants/analysis Receptors, Odorant/agonists,genetics,metabolism Smell/physiology Species Specificity Structure-Activity Relationship
Chemicals
Receptors, Odorant Luciferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Saito Harumi
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Research Drive, Durham, NC 27710, USA.
Chi Qiuyi
Zhuang Hanyi
Matsunami Hiroaki
Mainland Joel D
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Article Info
Journal
Science signaling
Abbr.
Sci Signal
ISSN
1937-9145
DOI
10.1126/scisignal.2000016
Published
2009-03-03
Epub
2009-00-03
Pages
ra9
Language
English
Region
United States
NLM ID
101465400
PMCID
PMC2774247
Subset
IM
Grants
NIDCD NIH HHS · R01 DC005782 · United States
NIDCD NIH HHS · R01 DC005782-05 · United States
NIDCD NIH HHS · R01 DC005782-02 · United States
NIDCD NIH HHS · F31 DC008480-01 · United States
NIDCD NIH HHS · R01 DC005782-03 · United States
NIDCD NIH HHS · R01 DC005782-06A1 · United States
NIDCD NIH HHS · F32 DC008932-02 · United States
NIDCD NIH HHS · F31 DC008480-02 · United States
NIDCD NIH HHS · F32 DC008932 · United States
NIDCD NIH HHS · R01 DC005782-04 · United States
NIDCD NIH HHS · F32 DC008932-01A2 · United States
NIDCD NIH HHS · R01 DC005782-01 · United States
External Links
PubMed source DOI: 10.1126/scisignal.2000016 PMC Full Text View journal details
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