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PMID: 19268588 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Regulation of Spo12 phosphorylation and its essential role in the FEAR network.

Current biology : CB ·Vol. 19 ·No. 6 ·2009-03-24 ·Pages 449-60

Tomson BN, Rahal R, Reiser V, Monje-Casas F, Mekhail K, Moazed D, Amon A

Abstract

In budding yeast, the protein phosphatase Cdc14 coordinates late mitotic events and triggers exit from mitosis. During early anaphase, Cdc14 is activated by the FEAR network, but how signaling through the FEAR network occurs is poorly understood. We find that the FEAR network component Spo12 is phosphorylated on S118. This phosphorylation is essential for Spo12 function and is restricted to early anaphase, when the FEAR network is active. The anaphase-specific phosphorylation of Spo12 requires mitotic CDKs and depends on the FEAR network components Separase and Slk19. Furthermore, we find that CDC14 is required to maintain Spo12 in the dephosphorylated state prior to anaphase. Our results show that anaphase-specific phosphorylation of Spo12 is essential for FEAR network function and raise the interesting possibility that Cdc14 itself helps to prevent the FEAR network from being prematurely activated.

MeSH Terms
Anaphase/physiology Cell Cycle Cell Cycle Proteins/metabolism DNA, Fungal/genetics DNA, Ribosomal/genetics Endopeptidases/metabolism Microtubule-Associated Proteins/metabolism Mitosis Mutation Nuclear Proteins/metabolism Phosphorylation Saccharomyces cerevisiae/cytology,metabolism,physiology Saccharomyces cerevisiae Proteins/metabolism Separase Spindle Apparatus/physiology
Chemicals
Cell Cycle Proteins DNA, Fungal DNA, Ribosomal Microtubule-Associated Proteins Nuclear Proteins SPO12 protein, S cerevisiae Saccharomyces cerevisiae Proteins Slk19 protein, S cerevisiae Endopeptidases ESP1 protein, S cerevisiae Separase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tomson Brett N
David H. Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
Rahal Rami
Reiser Vladimír
Monje-Casas Fernando
Mekhail Karim
Moazed Danesh
Amon Angelika
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Article Info
Journal
Current biology : CB
Abbr.
Curr Biol
ISSN
1879-0445
DOI
10.1016/j.cub.2009.02.024
Published
2009-03-24
Epub
2009-00-05
Pages
449-60
Language
English
Region
England
NLM ID
9107782
PMCID
PMC2692463
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R29 GM056800 · United States
NIGMS NIH HHS · R01 GM056800 · United States
NIGMS NIH HHS · GM056800 · United States
NIGMS NIH HHS · R01 GM056800-14 · United States
External Links
PubMed source DOI: 10.1016/j.cub.2009.02.024 PMC Full Text View journal details
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