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PMID: 19286647 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

ABCA1 plays no role in the centripetal movement of cholesterol from peripheral tissues to the liver and intestine in the mouse.

Journal of lipid research ·Vol. 50 ·No. 7 ·2009-07-00 ·Pages 1316-29

Xie C, Turley SD, Dietschy JM

Abstract

This study uses the mouse to explore the role of ABCA1 in the movement of this cholesterol from the peripheral organs to the endocrine glands for hormone synthesis and liver for excretion. The sterol pool in all peripheral organs was constant and equaled 2,218 and 2,269 mg/kg, respectively, in abca1(+/+) and abca1(-/-) mice. Flux of cholesterol from these tissues equaled the rate of synthesis plus the rate of LDL-cholesterol uptake and was 49.9 mg/day/kg in control animals and 62.0 mg/day/kg in abca1(-/-) mice. In the abca1(+/+) animals, this amount of cholesterol moved from HDL into the liver for excretion. In the abca1(-/-) mice, the cholesterol from the periphery also reached the liver but did not use HDL. Fecal excretion of cholesterol was just as high in abac1(-/-) mice (198 mg/day/kg) as in the abac1(+/+) animals (163 mg/day/kg), although the abac1(-/-) mice excreted relatively more neutral than acidic sterols. This study established that ABCA1 plays essentially no role in the turnover of cholesterol in peripheral organs or in the centripetal movement of this sterol to the endocrine glands, liver, and intestinal tract for excretion.

MeSH Terms
ATP Binding Cassette Transporter 1 ATP-Binding Cassette Transporters/genetics,metabolism Animals Cholesterol/metabolism Cholesterol, Dietary Cholesterol, HDL/metabolism Cholesterol, LDL/metabolism Feces/chemistry Female Hormones/biosynthesis,chemistry Intestinal Mucosa/metabolism Lipid Metabolism Liver/metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Organ Size Tissue Distribution
Chemicals
ATP Binding Cassette Transporter 1 ATP-Binding Cassette Transporters Cholesterol, Dietary Cholesterol, HDL Cholesterol, LDL Hormones Cholesterol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xie Chonglun
Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas, TX 75390-9151, USA.
Turley Stephen D
Dietschy John M
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Article Info
Journal
Journal of lipid research
Abbr.
J Lipid Res
ISSN
1539-7262
DOI
10.1194/jlr.M900024-JLR200
Published
2009-07-00
Epub
2009-00-12
Pages
1316-29
Language
English
Region
United States
NLM ID
0376606
PMCID
PMC2694331
Subset
IM
Grants
NHLBI NIH HHS · R01 HL09610 · United States
External Links
PubMed source DOI: 10.1194/jlr.M900024-JLR200 PMC Full Text View journal details
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