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PMID: 19332882 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Candida glabrata PHO4 is necessary and sufficient for Pho2-independent transcription of phosphate starvation genes.

Genetics ·Vol. 182 ·No. 2 ·2009-06-00 ·Pages 471-9

Kerwin CL, Wykoff DD

Abstract

Comparative genomic analyses of Candida glabrata and Saccharomyces cerevisiae suggest many signal transduction pathways are highly conserved. Focusing on the phosphate signal transduction (PHO) pathway of C. glabrata, we demonstrate that components of the pathway are conserved and confirm the role of CgPHO81, CgPHO80, CgPHO4, and CgMSN5 in the PHO pathway through deletion analysis. Unlike S. cerevisiae, C. glabrata shows little dependence on the transcription factor, Pho2, for induction of phosphate-regulated genes during phosphate limitation. We show that the CgPho4 protein is necessary and sufficient for Pho2-independent gene expression; CgPho4 is capable of driving expression of PHO promoters in S. cerevisiae in the absence of ScPHO2. On the basis of the sequences of PHO4 in the hemiascomycetes and complementation analysis, we suggest that Pho2 dependence is a trait only observed in species closely related to S. cerevisiae. Our data are consistent with trans-regulatory changes in the PHO pathway via the transcription factor Pho4 as opposed to cis-regulatory changes (the promoter).

MeSH Terms
Candida glabrata/genetics,metabolism Fungal Proteins/metabolism Genes, Fungal/genetics Genomics Phosphates/deficiency,metabolism Saccharomyces cerevisiae/genetics,metabolism Signal Transduction Transcription, Genetic
Chemicals
Fungal Proteins Phosphates
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kerwin Christine L
Department of Biology, Villanova University, Villanova, PA19085, USA.
Wykoff Dennis D
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.109.101063
Published
2009-06-00
Epub
2009-00-30
Pages
471-9
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC2691756
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.109.101063 PMC Full Text View journal details
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