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PMID: 19339686 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Nucleosome-binding affinity as a primary determinant of the nuclear mobility of the pioneer transcription factor FoxA.

Genes & development ·Vol. 23 ·No. 7 ·2009-04-01 ·Pages 804-9

Sekiya T, Muthurajan UM, Luger K, Tulin AV, Zaret KS

Abstract

FoxA proteins are pioneer transcription factors, among the first to bind chromatin domains in development and enable gene activity. The Fox DNA-binding domain structurally resembles linker histone and binds nucleosomes stably. Using fluorescence recovery after photobleaching, we found that FoxA1 and FoxA2 move much more slowly in nuclei than other transcription factor types, including c-Myc, GATA-4, NF-1, and HMGB1. We find that slower nuclear mobility correlates with high nonspecific nucleosome binding, and point mutations that disrupt nonspecific binding markedly increase nuclear mobility. FoxA's distinct nuclear mobility is consistent with its pioneer activity in chromatin.

MeSH Terms
Animals Cell Line Cell Nucleus/physiology Hepatocyte Nuclear Factor 3-alpha/genetics,metabolism,physiology Hepatocyte Nuclear Factor 3-beta/genetics,metabolism,physiology Mice Mutation Nucleosomes/metabolism Protein Binding/genetics Protein Transport Transcription Factors/physiology
Chemicals
Foxa1 protein, mouse Foxa2 protein, mouse Hepatocyte Nuclear Factor 3-alpha Nucleosomes Transcription Factors Hepatocyte Nuclear Factor 3-beta
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sekiya Takashi
Epigenetics and Progenitor Cells Program, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
Muthurajan Uma M
Luger Karolin
Tulin Alexei V
Zaret Kenneth S
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
1549-5477
DOI
10.1101/gad.1775509
Published
2009-04-01
Pages
804-9
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC2666343
Subset
IM
Grants
NIGMS NIH HHS · R01 GM077452 · United States
NIGMS NIH HHS · R01/R37GM36477 · United States
NIGMS NIH HHS · GM077452 · United States
NIGMS NIH HHS · R01 GM061909 · United States
NIGMS NIH HHS · R37 GM036477 · United States
NIGMS NIH HHS · GM061909 · United States
External Links
PubMed source DOI: 10.1101/gad.1775509 PMC Full Text View journal details
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