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PMID: 194059 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Circular Epstein-Barr virus genomes of reduced size in a human lymphoid cell line of infectious mononucleosis origin.

Journal of virology ·Vol. 22 ·No. 2 ·1977-05-00 ·Pages 373-80

Adams A, Bjursell G, Kaschka-Dierich C, Lindahl T

Abstract

Circular Epstein-Barr virus (EBV) DNA molecules have been purified and characterized from a human lymphoid cell line derived from a case of heterophile antibody-positive, blood transfusion-induced infectious mononucleosis, 883L. The circular EBV DNA in three cell lines obtained by transformation of human umbilical cord blood leukocytes with a strain of EBV originally derived from 883L was also studied. As estimated from sedimentation velocity data and electron microscopy, the circular EBV DNA molecules are 10 to 15% smaller than either the circular EBV DNA previously found intracellularly in several other types of EBV-transformed cells or the linear EBV DNA present extracellularly in virus particles. In addition, the EBV-transformed cord blood cell lines studied here differed from other EBV-transformed cells in that integrated virus DNA sequences could not be detected.

MeSH Terms
Burkitt Lymphoma Cell Line Cell Transformation, Neoplastic DNA, Circular/analysis DNA, Viral/analysis Fetal Blood Herpesvirus 4, Human/analysis,growth & development Humans Infectious Mononucleosis Leukocytes
Chemicals
DNA, Circular DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Adams A
Bjursell G
Kaschka-Dierich C
Lindahl T
References (36)
36 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.22.2.373-380.1977
Published
1977-05-00
Pages
373-80
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515728
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.22.2.373-380.1977 PMC Full Text View journal details
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