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PMID: 19411844 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Histone H3-K56 acetylation is important for genomic stability in mammals.

Cell cycle (Georgetown, Tex.) ·Vol. 8 ·No. 11 ·2009-06-01 ·Pages 1747-53

Yuan J, Pu M, Zhang Z, Lou Z

Abstract

Histone H3 lysine 56 acetylation (H3K56Ac) has recently been identified and shown to be important for genomic stability in yeast. However, whether or not H3K56 acetylation occurs in mammals is not clear. Here, we report that H3K56Ac exists in mammals. Mammalian H3K56Ac requires the histone chaperone Asf1 and occurs mainly at the S phase in unstressed cells. Moreover, SIRT1, which is a mammalian member of sirtuin family of NAD(+)-dependent deacetylases, regulates the deacetylation of H3K56. We further showed that proper H3K56 acetylation is critical for genomic stability and DNA damage response. These results establish the existence and functional significance of H3K56Ac in mammals and identify two regulators of this modification.

MeSH Terms
Acetylation Cell Cycle Proteins/metabolism Cell Line, Tumor DNA Damage Genomic Instability HeLa Cells Histones/metabolism Humans Lysine/metabolism Molecular Chaperones S Phase Sirtuin 1 Sirtuins/metabolism
Chemicals
ASF1A protein, human Cell Cycle Proteins Histones Molecular Chaperones SIRT1 protein, human Sirtuin 1 Sirtuins Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yuan Jian
Division of Oncology Research, Department of Oncology, Mayo Clinic, Rochester, MN 555902, USA.
Pu Mintie
Zhang Zhiguo
Lou Zhenkun
References (34)
34 references, click to expand
  1. DBC1 is a negative regulator of SIRT1.
    Nature. 2008 Jan 31;451(7178):583-6 PMID: 18235501
  2. Insights into the role of histone H3 and histone H4 core modifiable residues in Saccharomyces cerevisiae.
    Mol Cell Biol. 2005 Nov;25(22):10060-70 PMID: 16260619
  3. Histone H3 K56 acetylation, chromatin assembly, and the DNA damage checkpoint.
    DNA Repair (Amst). 2008 Dec 1;7(12):2020-4 PMID: 18848648
  4. The key to development: interpreting the histone code?
    Curr Opin Genet Dev. 2005 Apr;15(2):163-76 PMID: 15797199
  5. Cell cycle and checkpoint regulation of histone H3 K56 acetylation by Hst3 and Hst4.
    Mol Cell. 2006 Jul 7;23(1):109-19 PMID: 16818235
  6. Acetylated lysine 56 on histone H3 drives chromatin assembly after repair and signals for the completion of repair.
    Cell. 2008 Jul 25;134(2):231-43 PMID: 18662539
  7. Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56.
    Science. 2007 Feb 2;315(5812):649-52 PMID: 17272722
  8. The sirtuins hst3 and Hst4p preserve genome integrity by controlling histone h3 lysine 56 deacetylation.
    Curr Biol. 2006 Jul 11;16(13):1280-9 PMID: 16815704
  9. Organismal differences in post-translational modifications in histones H3 and H4.
    J Biol Chem. 2007 Mar 9;282(10):7641-55 PMID: 17194708
  10. Histones and histone modifications.
    Curr Biol. 2004 Jul 27;14(14):R546-51 PMID: 15268870
  11. A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage response.
    Nature. 2005 Jul 14;436(7048):294-8 PMID: 16015338
  12. Cooperation between complexes that regulate chromatin structure and transcription.
    Cell. 2002 Feb 22;108(4):475-87 PMID: 11909519
  13. Acetylation in histone H3 globular domain regulates gene expression in yeast.
    Cell. 2005 May 6;121(3):375-85 PMID: 15882620
  14. SIRT1 redistribution on chromatin promotes genomic stability but alters gene expression during aging.
    Cell. 2008 Nov 28;135(5):907-18 PMID: 19041753
  15. HST3/HST4-dependent deacetylation of lysine 56 of histone H3 in silent chromatin.
    Mol Biol Cell. 2008 Nov;19(11):4993-5005 PMID: 18799617
  16. Translating the histone code.
    Science. 2001 Aug 10;293(5532):1074-80 PMID: 11498575
  17. Impaired DNA damage response, genome instability, and tumorigenesis in SIRT1 mutant mice.
    Cancer Cell. 2008 Oct 7;14(4):312-23 PMID: 18835033
  18. Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair.
    Biochemistry. 2006 Mar 7;45(9):2852-61 PMID: 16503640
  19. Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactions.
    EMBO J. 2001 Sep 17;20(18):5207-18 PMID: 11566884
  20. Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication.
    Science. 2007 Feb 2;315(5812):653-5 PMID: 17272723
  21. Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3 K56 acetylation and Asf1.
    Mol Cell. 2007 Aug 3;27(3):393-405 PMID: 17679090
  22. Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly.
    Cell. 2008 Jul 25;134(2):244-55 PMID: 18662540
  23. Negative control of p53 by Sir2alpha promotes cell survival under stress.
    Cell. 2001 Oct 19;107(2):137-48 PMID: 11672522
  24. SIRT1 regulates the function of the Nijmegen breakage syndrome protein.
    Mol Cell. 2007 Jul 6;27(1):149-62 PMID: 17612497
  25. Acetylation of lysine 56 of histone H3 catalyzed by RTT109 and regulated by ASF1 is required for replisome integrity.
    J Biol Chem. 2007 Sep 28;282(39):28587-28596 PMID: 17690098
  26. SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin.
    Nature. 2008 Mar 27;452(7186):492-6 PMID: 18337721
  27. Structure and dynamic behavior of nucleosomes.
    Curr Opin Genet Dev. 2003 Apr;13(2):127-35 PMID: 12672489
  28. Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases.
    Curr Biol. 2001 Jul 10;11(13):1068-73 PMID: 11470414
  29. Histone H3-K56 acetylation is catalyzed by histone chaperone-dependent complexes.
    Mol Cell. 2007 Mar 9;25(5):703-12 PMID: 17320445
  30. Histone chaperone Asf1 is required for histone H3 lysine 56 acetylation, a modification associated with S phase in mitosis and meiosis.
    Proc Natl Acad Sci U S A. 2006 May 2;103(18):6988-93 PMID: 16627621
  31. Characterization of lysine 56 of histone H3 as an acetylation site in Saccharomyces cerevisiae.
    J Biol Chem. 2005 Jul 15;280(28):25949-52 PMID: 15888442
  32. The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability.
    Genes Dev. 1995 Dec 1;9(23):2888-902 PMID: 7498786
  33. Sirtuins in mammals: insights into their biological function.
    Biochem J. 2007 May 15;404(1):1-13 PMID: 17447894
  34. Histone H3 lysine 56 acetylation: a new twist in the chromosome cycle.
    Cell Cycle. 2006 Nov;5(22):2602-8 PMID: 17172838
Article Info
Journal
Cell cycle (Georgetown, Tex.)
Abbr.
Cell Cycle
ISSN
1551-4005
DOI
10.4161/cc.8.11.8620
Published
2009-06-01
Epub
2009-00-03
Pages
1747-53
Language
English
Region
United States
NLM ID
101137841
PMCID
PMC2776713
Subset
IM
Grants
NIGMS NIH HHS · R01 GM81838 · United States
NIGMS NIH HHS · R01 GM081838 · United States
NCI NIH HHS · R01 CA129344 · United States
NCI NIH HHS · R01 CA129344-01A1 · United States
NCI NIH HHS · CA 129344 · United States
Corrections
CommentIn
PMID: 19471124
External Links
PubMed source DOI: 10.4161/cc.8.11.8620 PMC Full Text View journal details
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