Home LiteratureArticle Details
PMID: 194241 Published · ppublish English Journal Article

In vitro synthesis of messenger RNA by a defective interfering particle of vesicular stomatitis virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 5 ·1977-05-00 ·Pages 1884-8

Colonno RJ, Lazzarini RA, Keene JD, Banerjee AK

Abstract

A defective interfering particle derived from the heat-resistant strain of vesicular stomatitis virus was analyzed for the presence of virion-associated RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) activtiy. The RNA synthesizing capacity of the defective particles in vitro was similar to that of the wild-type virus. Characterization of the RNA produced in vitro indicated that the defective particles were able to synthesize vesicular stomatitis virus leader RNA and four virus mRNA species that sediment at 12-18 S. These RNA products were identical to the mRNAs synthesized in vitro by the wild-type virus in regard to size, polyadenylation, capping, and methylation. In contrast to the wild-type virus, the purified defective particles did not synthesize the large mRNA species sedimenting at 31 S in vitro. Possible mechanisms of homotypic and heterotypic interferences shown by this defective particle are discussed.

MeSH Terms
Chromosome Mapping DNA-Directed RNA Polymerases/metabolism Defective Viruses/metabolism Genes Molecular Weight Poly A/biosynthesis RNA, Messenger/biosynthesis S-Adenosylmethionine/metabolism Vesicular stomatitis Indiana virus/metabolism
Chemicals
RNA, Messenger Poly A S-Adenosylmethionine DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Colonno R J
Lazzarini R A
Keene J D
Banerjee A K
References (30)
30 references, click to expand
  1. Homotypic non-exclusion by vesicular stomatitis virus in chick cell culture.
    J Gen Microbiol. 1958 Oct;19(2):350-64 PMID: 13587901
  2. Cell killing by viruses. II. Cell killing by vesicular stomatitis virus: a requirement for virion-derived transcription.
    Virology. 1975 Jan;63(1):176-90 PMID: 163045
  3. Messenger RNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus.
    Cell. 1975 Jan;4(1):37-43 PMID: 163702
  4. Translation and identification of the mRNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):274-8 PMID: 164017
  5. Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus.
    J Virol. 1975 Jun;15(6):1348-56 PMID: 167189
  6. Covalently linked message and anti-message (genomic) RNA from a defective vesicular stomatitis virus particle.
    J Mol Biol. 1975 Sep 25;97(3):289-307 PMID: 171415
  7. Order of transcription of genes of vesicular stomatitis virus.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):442-6 PMID: 174107
  8. The size and the cistronic origin of defective vesicular stomatitis virus particle RNAs in relation to homotypic and heterotypic interference.
    J Mol Biol. 1976 Mar 5;101(3):307-25 PMID: 176367
  9. Sequential transcription of the genes of vesicular stomatitis virus.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1504-8 PMID: 179088
  10. A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitro.
    Cell. 1976 Jun;8(2):197-204 PMID: 183891
  11. Analysis of the RNA species isolated from defective particles of vesicular stomatitis virus.
    J Gen Virol. 1976 Oct;33(1):51-60 PMID: 185328
  12. Vesicular stomatitis virus--a new interfering particle, intracellular structures, and virus-specific RNA.
    Virology. 1970 Aug;41(4):615-30 PMID: 4097494
  13. Defective particles in BHK cells infected with temperature-sensitive mutants of vesicular stomatitis virus.
    J Virol. 1971 Aug;8(2):154-60 PMID: 4107244
  14. Defective T particles of vesicular stomatitis virus. II. Biologic role in homologous interference.
    Virology. 1966 Oct;30(2):173-81 PMID: 4288277
  15. Structural proteins of vesicular stomatitis viruses.
    J Virol. 1969 Apr;3(4):395-403 PMID: 4306194
  16. Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens.
    J Virol. 1969 Apr;3(4):404-13 PMID: 4306195
  17. Homotypic and heterotypic interference by defective particles of vesicular stomatitis virus.
    Nature. 1970 Oct 3;228(5266):25-7 PMID: 4318492
  18. Ribonucleic acid synthesis of vesicular stomatitis virus. IV. Transcription by standard virus in the presence of defective interfering particles.
    J Virol. 1972 Jun;9(6):909-16 PMID: 4338638
  19. Genome homology of vesicular stomatitis virus and defective T particles and evidence for the sequential transcription of the virion ribonucleic acid.
    J Virol. 1972 Jun;9(6):946-55 PMID: 4338640
  20. Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions.
    J Virol. 1972 Aug;10(2):297-309 PMID: 4342247
  21. Absence of transcriptase activity or transcription-inhibiting ability in defective interfering particles of vesicular stomatitis virus.
    Virology. 1972 Oct;50(1):150-70 PMID: 4343109
  22. Mechanism of interferon action: inhibition of viral messenger ribonucleic acid translation in L-cell extracts.
    J Virol. 1972 Dec;10(6):1184-98 PMID: 4345494
  23. Defective interfering viruses.
    Annu Rev Microbiol. 1973;27:101-17 PMID: 4356530
  24. In vitro transcriptase activity of vesicular stomatitis virus B and T particles: analysis of product.
    Intervirology. 1973;1(3):168-75 PMID: 4359759
  25. Cell killing by viruses. I. Comparison of cell-killing, plaque-forming, and defective-interfering particles of vesicular stomatitis virus.
    Virology. 1974 Feb;57(2):321-38 PMID: 4361450
  26. RNA polymerase activity and poly(A) synthesizing activity in defective T particles of vesicular stomatitis virus.
    Virology. 1974 Mar;58(1):240-9 PMID: 4362548
  27. The RNA of defective vesicular stomatitis virus particles in relation to viral cistrons.
    J Mol Biol. 1974 Jan 5;85(4):551-68 PMID: 4369375
  28. Analysis of the RNA of defective VSV particles.
    Cell. 1974 Sep;3(1):85-93 PMID: 4369780
  29. Studies on the in vitro adenylation of RNA by vesicular stomatitis virus.
    Virology. 1974 Oct;61(2):547-58 PMID: 4371099
  30. Inhibition of viral transcriptase by immunoglobulin directed against the nucleocapsid NS protein of vesicular stomatitis virus.
    J Virol. 1975 Jun;15(6):1357-66 PMID: 49440
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.5.1884
Published
1977-05-00
Pages
1884-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431036
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.5.1884 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2