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PMID: 19439573 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The K-segment of maize DHN1 mediates binding to anionic phospholipid vesicles and concomitant structural changes.

Plant physiology ·Vol. 150 ·No. 3 ·2009-07-00 ·Pages 1503-14

Koag MC, Wilkens S, Fenton RD, Resnik J, Vo E, Close TJ

Abstract

Dehydrins (DHNs; late embryogenesis abundant D11 family) are a family of intrinsically unstructured plant proteins that accumulate in the late stages of seed development and in vegetative tissues subjected to water deficit, salinity, low temperature, or abscisic acid treatment. We demonstrated previously that maize (Zea mays) DHNs bind preferentially to anionic phospholipid vesicles; this binding is accompanied by an increase in alpha-helicity of the protein, and adoption of alpha-helicity can be induced by sodium dodecyl sulfate. All DHNs contain at least one "K-segment," a lysine-rich 15-amino acid consensus sequence. The K-segment is predicted to form a class A2 amphipathic alpha-helix, a structural element known to interact with membranes and proteins. Here, three K-segment deletion proteins of maize DHN1 were produced. Lipid vesicle-binding assays revealed that the K-segment is required for binding to anionic phospholipid vesicles, and adoption of alpha-helicity of the K-segment accounts for most of the conformational change of DHNs upon binding to anionic phospholipid vesicles or sodium dodecyl sulfate. The adoption of structure may help stabilize cellular components, including membranes, under stress conditions.

MeSH Terms
Amino Acid Sequence Consensus Sequence Cytoplasmic Vesicles/metabolism Escherichia coli/genetics Liposomes/metabolism Molecular Sequence Data Phospholipids/metabolism Plant Proteins/chemistry,genetics,physiology Protein Structure, Tertiary Seeds/growth & development,metabolism Sodium Dodecyl Sulfate/pharmacology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Zea mays/metabolism,ultrastructure
Chemicals
Liposomes Phospholipids Plant Proteins dehydrin proteins, plant Sodium Dodecyl Sulfate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Koag Myong-Chul
Graduate Program in Biochemistry and Molecular Biology, University of California, Riverside, California 92521-0124, USA.
Wilkens Stephan
Fenton Raymond D
Resnik Josh
Vo Evanly
Close Timothy J
References (63)
63 references, click to expand
  1. Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana.
    Plant Mol Biol. 2001 Feb;45(3):263-79 PMID: 11292073
  2. The calcium-binding activity of a vacuole-associated, dehydrin-like protein is regulated by phosphorylation.
    Plant Physiol. 2002 Oct;130(2):675-87 PMID: 12376635
  3. A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L.
    J Biol Chem. 2002 Jul 12;277(28):25062-9 PMID: 11983700
  4. Interaction of model class A1, class A2, and class Y amphipathic helical peptides with membranes.
    Biochemistry. 1996 Aug 27;35(34):11210-20 PMID: 8780526
  5. Protein-water and protein-buffer interactions in the aqueous solution of an intrinsically unstructured plant dehydrin: NMR intensity and DSC aspects.
    Biophys J. 2006 Sep 15;91(6):2243-9 PMID: 16798808
  6. Allelic variation of a dehydrin gene cosegregates with chilling tolerance during seedling emergence.
    Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13566-70 PMID: 10557361
  7. Structure and function of a mitochondrial late embryogenesis abundant protein are revealed by desiccation.
    Plant Cell. 2007 May;19(5):1580-9 PMID: 17526751
  8. A ca. 40 kDa maize (Zea mays L.) embryo dehydrin is encoded by the dhn2 locus on chromosome 9.
    Plant Mol Biol. 1998 Oct;38(3):417-23 PMID: 9747849
  9. Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco.
    Planta. 2003 Jun;217(2):290-8 PMID: 12783337
  10. Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers.
    Anal Chem. 1991 Dec 15;63(24):1193A-1203A PMID: 1789447
  11. The binding of maize DHN1 to lipid vesicles. Gain of structure and lipid specificity.
    Plant Physiol. 2003 Jan;131(1):309-16 PMID: 12529538
  12. Mimicking the plant cell interior under water stress by macromolecular crowding: disordered dehydrin proteins are highly resistant to structural collapse.
    Plant Physiol. 2008 Dec;148(4):1925-37 PMID: 18849483
  13. Intrinsically unstructured proteins and their functions.
    Nat Rev Mol Cell Biol. 2005 Mar;6(3):197-208 PMID: 15738986
  14. Purification and partial characterization of a dehydrin involved in chilling tolerance during seedling emergence of cowpea.
    Plant Physiol. 1999 May;120(1):237-44 PMID: 10318701
  15. A view of plant dehydrins using antibodies specific to the carboxy terminal peptide.
    Plant Mol Biol. 1993 Oct;23(2):279-86 PMID: 7693020
  16. Purification of recombinant Arabidopsis thaliana dehydrins by metal ion affinity chromatography.
    Protein Expr Purif. 2000 Nov;20(2):169-78 PMID: 11049741
  17. Cellular Concentrations and Uniformity of Cell-Type Accumulation of Two Lea Proteins in Cotton Embryos.
    Plant Cell. 1993 Jul;5(7):769-780 PMID: 12271086
  18. Modulation of Dehydration Tolerance in Soybean Seedlings (Dehydrin Mat1 Is Induced by Dehydration but Not by Abscisic Acid).
    Plant Physiol. 1997 Jul;114(3):917-925 PMID: 12223753
  19. Expression of SK3-type dehydrin in transporting organs is associated with cold acclimation in Solanum species.
    Planta. 2006 Jun;224(1):205-21 PMID: 16404580
  20. Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14570-5 PMID: 9826741
  21. Phosphorylation mediates the nuclear targeting of the maize Rab17 protein.
    Plant J. 1998 Mar;13(5):691-7 PMID: 9681011
  22. Transition from natively unfolded to folded state induced by desiccation in an anhydrobiotic nematode protein.
    J Biol Chem. 2003 Apr 11;278(15):12977-84 PMID: 12569097
  23. Metal binding by citrus dehydrin with histidine-rich domains.
    J Exp Bot. 2005 Oct;56(420):2695-703 PMID: 16131509
  24. Dehydration-induced conformational transitions in proteins and their inhibition by stabilizers.
    Biophys J. 1993 Aug;65(2):661-71 PMID: 7693001
  25. What does it mean to be natively unfolded?
    Eur J Biochem. 2002 Jan;269(1):2-12 PMID: 11784292
  26. Lipid polymorphism and the roles of lipids in membranes.
    Chem Phys Lipids. 1986 Jun-Jul;40(2-4):127-44 PMID: 3742670
  27. An important role of phosphatidic acid in ABA signaling during germination in Arabidopsis thaliana.
    Plant J. 2005 Jul;43(1):107-17 PMID: 15960620
  28. NMR relaxation studies on the hydrate layer of intrinsically unstructured proteins.
    Biophys J. 2005 Mar;88(3):2030-7 PMID: 15613629
  29. Signal transduction and membrane traffic: the PITP/phosphoinositide connection.
    Cell. 1995 Jun 2;81(5):659-62 PMID: 7774006
  30. Structural investigation of disordered stress proteins. Comparison of full-length dehydrins with isolated peptides of their conserved segments.
    Plant Physiol. 2006 Jun;141(2):638-50 PMID: 16565295
  31. Intrinsically unstructured proteins: re-assessing the protein structure-function paradigm.
    J Mol Biol. 1999 Oct 22;293(2):321-31 PMID: 10550212
  32. Expression of KS-type dehydrins is primarily regulated by factors related to organ type and leaf developmental stage during vegetative growth.
    Planta. 2004 Mar;218(5):878-85 PMID: 14685858
  33. Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis.
    Plant Mol Biol. 2004 Mar;54(5):743-53 PMID: 15356392
  34. Production of large unilamellar vesicles by a rapid extrusion procedure: characterization of size distribution, trapped volume and ability to maintain a membrane potential.
    Biochim Biophys Acta. 1985 Jan 10;812(1):55-65 PMID: 23008845
  35. Photoperiod and temperature interactions regulate low-temperature-induced gene expression in barley.
    Plant Physiol. 2001 Dec;127(4):1676-81 PMID: 11743112
  36. Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance.
    Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13404-9 PMID: 11038526
  37. Expression of the barley dehydrin multigene family and the development of freezing tolerance.
    Mol Gen Genet. 2000 Sep;264(1-2):145-53 PMID: 11016844
  38. The interplay between structure and function in intrinsically unstructured proteins.
    FEBS Lett. 2005 Jun 13;579(15):3346-54 PMID: 15943980
  39. Immunolocalization of freezing-tolerance-associated proteins in the cytoplasm and nucleoplasm of wheat crown tissues.
    Plant J. 1995 Oct;8(4):583-93 PMID: 7496403
  40. Gene cloning and characterization of a soybean (Glycine max L.) LEA protein, GmPM16.
    Plant Mol Biol. 2004 Nov;56(5):689-703 PMID: 15803408
  41. Conformation of a group 2 late embryogenesis abundant protein from soybean. Evidence of poly (L-proline)-type II structure.
    Plant Physiol. 2003 Mar;131(3):963-75 PMID: 12644649
  42. Mechanisms for the modulation of membrane bilayer properties by amphipathic helical peptides.
    Biopolymers. 1995;37(5):319-38 PMID: 7632881
  43. Dehydrin gene expression provides an indicator of low temperature and drought stress: transcriptome-based analysis of barley (Hordeum vulgare L.).
    Funct Integr Genomics. 2008 Nov;8(4):387-405 PMID: 18512091
  44. The recombinant dehydrin-like desiccation stress protein from the resurrection plant Craterostigma plantagineum displays no defined three-dimensional structure in its native state.
    Biol Chem. 1996 Sep;377(9):555-61 PMID: 9067253
  45. Ion binding properties of the dehydrin ERD14 are dependent upon phosphorylation.
    J Biol Chem. 2003 Oct 17;278(42):40882-9 PMID: 12917402
  46. Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat
    Plant Cell. 1998 Apr;10(4):623-38 PMID: 9548987
  47. Barley Dhn13 encodes a KS-type dehydrin with constitutive and stress responsive expression.
    Theor Appl Genet. 2005 Mar;110(5):852-8 PMID: 15711789
  48. Chaperone activity of ERD10 and ERD14, two disordered stress-related plant proteins.
    Plant Physiol. 2008 May;147(1):381-90 PMID: 18359842
  49. Water deficit triggers phospholipase D activity in the resurrection plant Craterostigma plantagineum.
    Plant Cell. 2000 Jan;12(1):111-24 PMID: 10634911
  50. Purification of a maize dehydrin protein expressed in Escherichia coli.
    Protein Expr Purif. 1995 Oct;6(5):632-6 PMID: 8535156
  51. Charge environments around phosphorylation sites in proteins.
    BMC Struct Biol. 2008 Mar 25;8:19 PMID: 18366741
  52. Expression, tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants.
    Plant Mol Biol. 1994 Dec;26(6):1921-34 PMID: 7858227
  53. Intrinsically unstructured proteins.
    Trends Biochem Sci. 2002 Oct;27(10):527-33 PMID: 12368089
  54. DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data.
    Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W668-73 PMID: 15215473
  55. Binding of nucleotides to guanylate kinase, p21(ras), and nucleoside-diphosphate kinase studied by nano-electrospray mass spectrometry.
    J Biol Chem. 1999 Dec 10;274(50):35337-42 PMID: 10585400
  56. Comparing and combining predictors of mostly disordered proteins.
    Biochemistry. 2005 Feb 15;44(6):1989-2000 PMID: 15697224
  57. Calculation of protein extinction coefficients from amino acid sequence data.
    Anal Biochem. 1989 Nov 1;182(2):319-26 PMID: 2610349
  58. Thermodynamics and stability of the PAAD/DAPIN/PYRIN domain of IFI-16.
    FEBS Lett. 2006 May 29;580(13):3083-90 PMID: 16678172
  59. How membranes shape protein structure.
    J Biol Chem. 2001 Aug 31;276(35):32395-8 PMID: 11432876
  60. Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit.
    J Biol Chem. 2000 Feb 25;275(8):5668-74 PMID: 10681550
  61. Chloroplast biogenesis. Regulation of lipid transport to the thylakoid in chloroplasts isolated from expanding and fully expanded leaves of pea.
    Plant Physiol. 2001 Sep;127(1):184-93 PMID: 11553746
  62. Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography.
    Anal Biochem. 1993 Jun;211(2):279-87 PMID: 8317704
  63. THE MOLECULAR BASIS OF DEHYDRATION TOLERANCE IN PLANTS.
    Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:377-403 PMID: 15012294
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.109.136697
Published
2009-07-00
Epub
2009-00-13
Pages
1503-14
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2705017
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.109.136697 PMC Full Text View journal details
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