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PMID: 19439651 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human memory FOXP3+ Tregs secrete IL-17 ex vivo and constitutively express the T(H)17 lineage-specific transcription factor RORgamma t.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 106 ·No. 21 ·2009-05-26 ·Pages 8635-40

Ayyoub M, Deknuydt F, Raimbaud I, Dousset C, Leveque L, Bioley G, Valmori D

Abstract

Recent studies have suggested a close relationship between CD4(+)FOXP3(+) regulatory T cells (Tregs) and proinflammatory IL-17-producing T helper cells (T(H)17) expressing the lineage-specific transcription factor RORgamma t. We report here the unexpected finding that human memory Tregs secrete IL-17 ex vivo and constitutively express RORgamma t. IL-17-secreting Tregs share some phenotypic and functional features with conventional T(H)17 cells, expressing high levels of CCR4 and CCR6 and low levels of CXCR3. However, unlike conventional T(H)17 cells, they express low levels of CD161 and mostly fail to cosecrete IL-22 and TNF-alpha ex vivo. Ex vivo secretion of IL-17 and constitutive expression of RORgamma t by human memory Tregs suggest that, in addition to their well-known suppressive functions, these cells likely play additional, as yet undescribed, proinflammatory functions.

MeSH Terms
Cell Lineage/immunology Forkhead Transcription Factors/immunology Humans Immunologic Memory/immunology Interleukin-17/immunology,metabolism Nuclear Receptor Subfamily 1, Group F, Member 3 Phenotype Receptors, Retinoic Acid/immunology Receptors, Thyroid Hormone/immunology T-Lymphocytes, Helper-Inducer/immunology T-Lymphocytes, Regulatory/immunology,metabolism
Chemicals
FOXP3 protein, human Forkhead Transcription Factors Interleukin-17 Nuclear Receptor Subfamily 1, Group F, Member 3 RORC protein, human Receptors, Retinoic Acid Receptors, Thyroid Hormone
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ayyoub Maha
Institut National de la Santé et de la Recherche Médicale, Unité 892, Centre René Gauducheau, 44800 Saint Herblain, France. [email protected]
Deknuydt Florence
Raimbaud Isabelle
Dousset Christelle
Leveque Lucie
Bioley Gilles
Valmori Danila
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0900621106
Email
[email protected]
Published
2009-05-26
Epub
2009-00-13
Pages
8635-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2688993
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0900621106 PMC Full Text View journal details
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