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PMID: 19465906 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology ·Vol. 10 ·No. 7 ·2009-07-00 ·Pages 697-705

Perrigoue JG, Saenz SA, Siracusa MC, Allenspach EJ, Taylor BC, Giacomin PR, Nair MG, Du Y, Zaph C, van Rooijen N, Comeau MR, Pearce EJ, Laufer TM, Artis D

Abstract

Dendritic cells can prime naive CD4+ T cells; however, here we demonstrate that dendritic cell-mediated priming was insufficient for the development of T helper type 2 cell-dependent immunity. We identify basophils as a dominant cell population that coexpressed major histocompatibility complex class II and interleukin 4 message after helminth infection. Basophilia was promoted by thymic stromal lymphopoietin, and depletion of basophils impaired immunity to helminth infection. Basophils promoted antigen-specific CD4+ T cell proliferation and interleukin 4 production in vitro, and transfer of basophils augmented the population expansion of helminth-responsive CD4+ T cells in vivo. Collectively, our studies suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.

MeSH Terms
Animals Basophils/cytology,immunology,metabolism CD11c Antigen/immunology CD4-Positive T-Lymphocytes/cytology,immunology,metabolism Cell Communication/immunology Cell Proliferation Cytokines/immunology,metabolism Female Flow Cytometry Gene Expression Histocompatibility Antigens Class II/genetics,immunology Immunity/immunology Immunoblotting Interleukin-4/genetics,metabolism Male Mice Mice, Inbred C57BL Mice, Inbred Strains Mice, Knockout Reverse Transcriptase Polymerase Chain Reaction Schistosomiasis mansoni/immunology,parasitology Th2 Cells/metabolism Thymus Gland/cytology,immunology Trichuriasis/immunology,parasitology
Chemicals
CD11c Antigen Cytokines Histocompatibility Antigens Class II Interleukin-4
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Perrigoue Jacqueline G
Department of Pathobiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Saenz Steven A
Siracusa Mark C
Allenspach Eric J
Taylor Betsy C
Giacomin Paul R
Nair Meera G
Du Yurong
Zaph Colby
van Rooijen Nico
Comeau Michael R
Pearce Edward J
Laufer Terri M
Artis David
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Article Info
Journal
Nature immunology
Abbr.
Nat Immunol
ISSN
1529-2916
DOI
10.1038/ni.1740
Published
2009-07-00
Epub
2009-00-24
Pages
697-705
Language
English
Region
United States
NLM ID
100941354
PMCID
PMC2711559
Subset
IM
Grants
NIAID NIH HHS · R01 AI074878 · United States
NIAID NIH HHS · R56 AI032573 · United States
NCI NIH HHS · T32 CA09140-30 · United States
NIDDK NIH HHS · DK50306 · United States
NIAID NIH HHS · AI074878 · United States
NIGMS NIH HHS · F31 GM082187 · United States
NIAID NIH HHS · R01 AI074878-02 · United States
NIAID NIH HHS · R01 AI061570 · United States
NIAID NIH HHS · AI61570 · United States
NIDDK NIH HHS · P30 DK050306 · United States
NIAID NIH HHS · T32 AI007532 · United States
NIAID NIH HHS · T32 AI007532-08 · United States
NIAID NIH HHS · R01 AI053825 · United States
NIAID NIH HHS · R01 AI032573 · United States
NIAID NIH HHS · AI32573 · United States
NIAID NIH HHS · R01 AI061570-05 · United States
NIAID NIH HHS · AI53825 · United States
External Links
PubMed source DOI: 10.1038/ni.1740 PMC Full Text View journal details
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