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PMID: 19516020 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

A novel, single, transmembrane protein CATSPERG is associated with CATSPER1 channel protein.

Biology of reproduction ·Vol. 81 ·No. 3 ·2009-09-00 ·Pages 539-44

Wang H, Liu J, Cho KH, Ren D

Abstract

All four CATSPER channel pore-forming subunits (CATSPER1-4) are localized in the sperm principal piece. They form an alkalization-activated Ca2+-permeable channel and are required for sperm-hyperactivated motility, egg coat penetration, and male fertility. Unlike many other ion channels, the composition of the CATSPER protein complex is poorly defined. Herein, we describe the novel protein CATSPERG associated with the CATSPER complex. CATSPERG is predicted to be a single transmembrane-spanning protein with a large extracellular domain and a short intracellular tail. Like all the CATSPERs and the previously identified CATSPER-associated protein CATSPERB, CATSPERG is only expressed in testis and is localized in the sperm principal piece. In CATSPER1-deficient sperm, the CATSPERG protein (but not the K+ channel protein KCNU1) is also lost. Together with previous findings, our data suggest that the CATSPER protein complex contains pore-forming proteins and two additional proteins (CATSPERB and CATSPERG) and that the trafficking and/or assembly of these proteins depends on CATSPER1.

MeSH Terms
Amino Acid Sequence Animals Calcium Channels/metabolism Cloning, Molecular Male Membrane Proteins/chemistry,genetics,metabolism Mice Mice, Transgenic Models, Biological Molecular Sequence Data Protein Binding Spermatozoa/metabolism Testis/metabolism Tissue Distribution
Chemicals
Calcium Channels Catsper1 protein, mouse Catsperg2 protein, mouse Membrane Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wang Haikun
Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Liu Jin
Cho Kwang-Hyun
Ren Dejian
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Article Info
Journal
Biology of reproduction
Abbr.
Biol Reprod
ISSN
1529-7268
DOI
10.1095/biolreprod.109.077107
Published
2009-09-00
Epub
2009-00-10
Pages
539-44
Language
English
Region
United States
NLM ID
0207224
PMCID
PMC2731986
Subset
IM
Grants
NICHD NIH HHS · R01 HD047578 · United States
NICHD NIH HHS · R03 HD045290 · United States
NICHD NIH HHS · 1R03HD045290 · United States
NICHD NIH HHS · 1R01HD047578 · United States
External Links
PubMed source DOI: 10.1095/biolreprod.109.077107 PMC Full Text View journal details
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