Home LiteratureArticle Details
PMID: 19529823 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A major role of the MEKK1-MKK1/2-MPK4 pathway in ROS signalling.

Molecular plant ·Vol. 2 ·No. 1 ·2009-01-00 ·Pages 120-37

Pitzschke A, Djamei A, Bitton F, Hirt H

Abstract

Over the last few years, it has become evident that reactive oxygen species (ROS) signalling plays an important role in various physiological responses, including pathogen defense and stomatal opening/closure. On the other hand, ROS overproduction is detrimental for proper plant growth and development, indicating that the regulation of an appropriate redox balance is essential for plants. ROS homeostasis in plants involves the mitogen-activated protein kinase (MAPK) pathway consisting of the MAPK kinase kinase MEKK1 and the MAPK MPK4. Phenotypic and molecular analysis revealed that the MAPK kinases MKK1 and MKK2 are part of a cascade, regulating ROS and salicylic acid (SA) accumulation. Gene expression analysis shows that of 32 transcription factors reported to be highly responsive to multiple ROS-inducing conditions, 20 are regulated by the MEKK1, predominantly via the MEKK1-MKK1/2-MPK4 pathway. However, MEKK1 also functions on other as yet unknown pathways and part of the MEKK1-dependent MPK4 responses are regulated independently of MKK1 and MKK2. Overall, this analysis emphasizes the central role of this MAPK cascade in oxidative stress signalling, but also indicates the high level of complexity revealed by this signalling network.

Keywords
MEKK1 MKK1 MKK2 MPK4 Mitogen-activated protein kinases differential gene expression reactive oxygen species redox homeostasis stress signalling
MeSH Terms
Gene Expression Profiling Genes, Plant MAP Kinase Signaling System Photosynthesis Reactive Oxygen Species/metabolism Signal Transduction
Chemicals
Reactive Oxygen Species
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pitzschke Andrea
Department of Plant Molecular Biology, Max F. Perutz Laboratories, University of Vienna, Dr.-Bohr-Gasse 9, 1030 Vienna, Austria.
Djamei Armin
Bitton Frédérique
Hirt Heribert
References (47)
47 references, click to expand
  1. Distinct regulation of salinity and genotoxic stress responses by Arabidopsis MAP kinase phosphatase 1.
    EMBO J. 2002 Dec 2;21(23):6483-93 PMID: 12456655
  2. OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis.
    Nature. 2004 Feb 26;427(6977):858-61 PMID: 14985766
  3. Members of the Arabidopsis WRKY group III transcription factors are part of different plant defense signaling pathways.
    Mol Plant Microbe Interact. 2003 Apr;16(4):295-305 PMID: 12744458
  4. Senescence-specific regulation of catalases in Arabidopsis thaliana (L.) Heynh.
    Plant Cell Environ. 2006 Jun;29(6):1049-60 PMID: 17080932
  5. Bacteria-derived peptidoglycans constitute pathogen-associated molecular patterns triggering innate immunity in Arabidopsis.
    J Biol Chem. 2007 Nov 2;282(44):32338-48 PMID: 17761682
  6. Nicotiana benthamiana gp91phox homologs NbrbohA and NbrbohB participate in H2O2 accumulation and resistance to Phytophthora infestans.
    Plant Cell. 2003 Mar;15(3):706-18 PMID: 12615943
  7. Activation of a mitogen-activated protein kinase pathway in Arabidopsis by chitin.
    Mol Plant Pathol. 2004 Mar 1;5(2):125-35 PMID: 20565589
  8. CATMA: a complete Arabidopsis GST database.
    Nucleic Acids Res. 2003 Jan 1;31(1):156-8 PMID: 12519971
  9. Arabidopsis MEKK1 can take a short cut: it can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter.
    Plant Mol Biol. 2007 Sep;65(1-2):63-76 PMID: 17587183
  10. Versatile gene-specific sequence tags for Arabidopsis functional genomics: transcript profiling and reverse genetics applications.
    Genome Res. 2004 Oct;14(10B):2176-89 PMID: 15489341
  11. CATdb: a public access to Arabidopsis transcriptome data from the URGV-CATMA platform.
    Nucleic Acids Res. 2008 Jan;36(Database issue):D986-90 PMID: 17940091
  12. Multiple mediators of plant programmed cell death: interplay of conserved cell death mechanisms and plant-specific regulators.
    Bioessays. 2003 Jan;25(1):47-57 PMID: 12508282
  13. Isolation of ATMEKK1 (a MAP kinase kinase kinase)-interacting proteins and analysis of a MAP kinase cascade in Arabidopsis.
    Biochem Biophys Res Commun. 1998 Dec 18;253(2):532-43 PMID: 9878570
  14. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response.
    Plant Mol Biol. 2003 Jan;51(1):21-37 PMID: 12602888
  15. MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants.
    Plant Physiol. 2007 Feb;143(2):661-9 PMID: 17142480
  16. The tobacco wounding-activated mitogen-activated protein kinase is encoded by SIPK.
    Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7225-30 PMID: 9618567
  17. OMTK1, a novel MAPKKK, channels oxidative stress signaling through direct MAPK interaction.
    J Biol Chem. 2004 Jun 25;279(26):26959-66 PMID: 15033984
  18. Pathogen derived elicitors: searching for receptors in plants.
    Mol Plant Pathol. 2003 Jan 1;4(1):73-9 PMID: 20569365
  19. Mitogen-activated protein kinases and reactive oxygen species signaling in plants.
    Plant Physiol. 2006 Jun;141(2):351-6 PMID: 16760487
  20. Integration of Arabidopsis thaliana stress-related transcript profiles, promoter structures, and cell-specific expression.
    Genome Biol. 2007;8(4):R49 PMID: 17408486
  21. Arabidopsis map kinase 4 negatively regulates systemic acquired resistance.
    Cell. 2000 Dec 22;103(7):1111-20 PMID: 11163186
  22. Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat-induced photooxidative stress and to nitric oxide-induced cell death.
    Plant J. 2004 Jun;38(6):940-53 PMID: 15165186
  23. Phosphorylation of 1-aminocyclopropane-1-carboxylic acid synthase by MPK6, a stress-responsive mitogen-activated protein kinase, induces ethylene biosynthesis in Arabidopsis.
    Plant Cell. 2004 Dec;16(12):3386-99 PMID: 15539472
  24. A Mitogen-activated protein kinase kinase kinase mediates reactive oxygen species homeostasis in Arabidopsis.
    J Biol Chem. 2006 Dec 15;281(50):38697-704 PMID: 17043356
  25. Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis.
    Plant Cell. 2004 Aug;16(8):2089-103 PMID: 15269332
  26. Cross talk in defense signaling.
    Plant Physiol. 2008 Mar;146(3):839-44 PMID: 18316638
  27. Arabidopsis mitogen-activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1.
    Plant Physiol. 2008 Sep;148(1):212-22 PMID: 18599650
  28. Overexpression of Arabidopsis MAP kinase kinase 7 leads to activation of plant basal and systemic acquired resistance.
    Plant J. 2007 Dec;52(6):1066-79 PMID: 17908155
  29. Structure, expression, and antisense inhibition of the systemin precursor gene.
    Science. 1992 Mar 20;255(5051):1570-3 PMID: 1549783
  30. Functional characterization of ARAKIN (ATMEKK1): a possible mediator in an osmotic stress response pathway in higher plants.
    Biochim Biophys Acta. 1999 Sep 21;1451(2-3):242-54 PMID: 10556579
  31. Microbial elicitors induce activation and dual phosphorylation of the Arabidopsis thaliana MAPK 6.
    J Biol Chem. 2000 Mar 17;275(11):7521-6 PMID: 10713056
  32. Emerging MAP kinase pathways in plant stress signalling.
    Trends Plant Sci. 2005 Jul;10(7):339-46 PMID: 15953753
  33. The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin.
    Plant J. 2006 Nov;48(4):485-98 PMID: 17059410
  34. Mechanical stress induces biotic and abiotic stress responses via a novel cis-element.
    PLoS Genet. 2007 Oct;3(10):1800-12 PMID: 17953483
  35. A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):765-9 PMID: 8570631
  36. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants.
    Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2940-5 PMID: 10717008
  37. Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances paraquat-induced photooxidative stress and nitric oxide-induced cell death.
    Planta. 2005 Aug;221(6):757-65 PMID: 15744494
  38. The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis.
    Mol Cell. 2004 Jul 2;15(1):141-52 PMID: 15225555
  39. Cell death mediated by MAPK is associated with hydrogen peroxide production in Arabidopsis.
    J Biol Chem. 2002 Jan 4;277(1):559-65 PMID: 11687590
  40. Coordinated plant defense responses in Arabidopsis revealed by microarray analysis.
    Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11655-60 PMID: 11027363
  41. Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis.
    Plant Mol Biol. 2004 Aug;55(6):853-67 PMID: 15604721
  42. Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis.
    Plant Physiol. 2006 Jun;141(2):436-45 PMID: 16603662
  43. Reactive oxygen gene network of plants.
    Trends Plant Sci. 2004 Oct;9(10):490-8 PMID: 15465684
  44. Chloroplast-generated reactive oxygen species are involved in hypersensitive response-like cell death mediated by a mitogen-activated protein kinase cascade.
    Plant J. 2007 Sep;51(6):941-54 PMID: 17651371
  45. Catalase deficiency drastically affects gene expression induced by high light in Arabidopsis thaliana.
    Plant J. 2004 Jul;39(1):45-58 PMID: 15200641
  46. MAP kinase signalling cascade in Arabidopsis innate immunity.
    Nature. 2002 Feb 28;415(6875):977-83 PMID: 11875555
  47. MEKK1 is required for MPK4 activation and regulates tissue-specific and temperature-dependent cell death in Arabidopsis.
    J Biol Chem. 2006 Dec 1;281(48):36969-76 PMID: 17023433
Article Info
Journal
Molecular plant
Abbr.
Mol Plant
ISSN
1674-2052
DOI
10.1093/mp/ssn079
Published
2009-01-00
Epub
2009-00-06
Pages
120-37
Language
English
Region
England
NLM ID
101465514
PMCID
PMC2639734
Subset
IM
External Links
PubMed source DOI: 10.1093/mp/ssn079 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2