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PMID: 19529832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heat shock factors HsfB1 and HsfB2b are involved in the regulation of Pdf1.2 expression and pathogen resistance in Arabidopsis.

Molecular plant ·Vol. 2 ·No. 1 ·2009-01-00 ·Pages 152-65

Kumar M, Busch W, Birke H, Kemmerling B, Nürnberger T, Schöffl F

Abstract

In order to assess the functional roles of heat stress-induced class B-heat shock factors in Arabidopsis, we investigated T-DNA knockout mutants of AtHsfB1 and AtHsfB2b. Micorarray analysis of double knockout hsfB1/hsfB2b plants revealed as strong an up-regulation of the basal mRNA-levels of the defensin genes Pdf1.2a/b in mutant plants. The Pdf expression was further enhanced by jasmonic acid treatment or infection with the necrotrophic fungus Alternaria brassicicola. The single mutant hsfB2b and the double mutant hsfB1/B2b were significantly improved in disease resistance after A. brassicicola infection. There was no indication for a direct interaction of Hsf with the promoter of Pdf1.2, which is devoid of perfect HSE consensus Hsf-binding sequences. However, changes in the formation of late HsfA2-dependent HSE binding were detected in hsfB1/B2b plants. This suggests that HsfB1/B2b may interact with class A-Hsf in regulating the shut-off of the heat shock response. The identification of Pdf genes as targets of Hsf-dependent negative regulation is the first evidence for an interconnection of Hsf in the regulation of biotic and abiotic responses.

Keywords
Abiotic/environmental stress Arabidopsis defense responses disease resistance transcriptional control and transcription factors transcriptome analysis
MeSH Terms
Arabidopsis/genetics,microbiology,physiology Arabidopsis Proteins/genetics Base Sequence DNA Primers Defensins/genetics Electrophoretic Mobility Shift Assay Gene Expression Profiling Gene Expression Regulation, Plant/genetics Gene Knockout Techniques Genes, Plant Heat-Shock Proteins/physiology Polymerase Chain Reaction
Chemicals
Arabidopsis Proteins DNA Primers Defensins Heat-Shock Proteins PDF1.2 protein, Arabidopsis
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kumar Mukesh
Heinrich-Pette-Institut, Martinistrabe 52, D-20251 Hamburg, Germany.
Busch Wolfgang
Birke Hannah
Kemmerling Birgit
Nürnberger Thorsten
Schöffl Friedrich
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Article Info
Journal
Molecular plant
Abbr.
Mol Plant
ISSN
1674-2052
DOI
10.1093/mp/ssn095
Published
2009-01-00
Pages
152-65
Language
English
Region
England
NLM ID
101465514
PMCID
PMC2639743
Subset
IM
External Links
PubMed source DOI: 10.1093/mp/ssn095 PMC Full Text View journal details
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