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PMID: 19570907 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Budding yeast SSD1-V regulates transcript levels of many longevity genes and extends chronological life span in purified quiescent cells.

Molecular biology of the cell ·Vol. 20 ·No. 17 ·2009-09-00 ·Pages 3851-64

Li L, Lu Y, Qin LX, Bar-Joseph Z, Werner-Washburne M, Breeden LL

Abstract

Ssd1 is an RNA-binding protein that affects literally hundreds of different processes and is polymorphic in both wild and lab yeast strains. We have used transcript microarrays to compare mRNA levels in an isogenic pair of mutant (ssd1-d) and wild-type (SSD1-V) cells across the cell cycle. We find that 15% of transcripts are differentially expressed, but there is no correlation with those mRNAs bound by Ssd1. About 20% of cell cycle regulated transcripts are affected, and most show sharper amplitudes of oscillation in SSD1-V cells. Many transcripts whose gene products influence longevity are also affected, the largest class of which is involved in translation. Ribosomal protein mRNAs are globally down-regulated by SSD1-V. SSD1-V has been shown to increase replicative life span currency and we show that SSD1-V also dramatically increases chronological life span (CLS). Using a new assay of CLS in pure populations of quiescent prototrophs, we find that the CLS for SSD1-V cells is twice that of ssd1-d cells.

MeSH Terms
Cell Cycle/physiology Cluster Analysis Gene Expression Profiling Gene Expression Regulation, Fungal Longevity/genetics Oligonucleotide Array Sequence Analysis Polymorphism, Genetic Protein Isoforms/genetics,metabolism Ribosomal Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Protein Isoforms Ribosomal Proteins Saccharomyces cerevisiae Proteins Ssd1 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Li Lihong
Fred Hutchinson Cancer Research Center, Basic Sciences Division, Seattle, WA 98109, USA.
Lu Yong
Qin Li-Xuan
Bar-Joseph Ziv
Werner-Washburne Margaret
Breeden Linda L
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e09-04-0347
Published
2009-09-00
Epub
2009-00-01
Pages
3851-64
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2735484
Subset
IM
Grants
NCI NIH HHS · P30 CA015704 · United States
NIGMS NIH HHS · R01 GM041073 · United States
NIGMS NIH HHS · GM41073 · United States
External Links
PubMed source DOI: 10.1091/mbc.e09-04-0347 PMC Full Text View journal details
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