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PMID: 19654187 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Tau fragmentation, aggregation and clearance: the dual role of lysosomal processing.

Human molecular genetics ·Vol. 18 ·No. 21 ·2009-11-01 ·Pages 4153-70

Wang Y, Martinez-Vicente M, Krüger U, Kaushik S, Wong E, Mandelkow EM, Cuervo AM, Mandelkow E

Abstract

Aggregation and cleavage are two hallmarks of Tau pathology in Alzheimer disease (AD), and abnormal fragmentation of Tau is thought to contribute to the nucleation of Tau paired helical filaments. Clearance of the abnormally modified protein could occur by the ubiquitin-proteasome and autophagy-lysosomal pathways, the two major routes for protein degradation in cells. There is a debate on which of these pathways contributes to clearance of Tau protein and of the abnormal Tau aggregates formed in AD. Here, we demonstrate in an inducible neuronal cell model of tauopathy that the autophagy-lysosomal system contributes to both Tau fragmentation into pro-aggregating forms and to clearance of Tau aggregates. Inhibition of macroautophagy enhances Tau aggregation and cytotoxicity. The Tau repeat domain can be cleaved near the N terminus by a cytosolic protease to generate the fragment F1. Additional cleavage near the C terminus by the lysosomal protease cathepsin L is required to generate Tau fragments F2 and F3 that are highly amyloidogenic and capable of seeding the aggregation of Tau. We identify in this work that components of a selective form of autophagy, chaperone-mediated autophagy, are involved in the delivery of cytosolic Tau to lysosomes for this limited cleavage. However, F1 does not fully enter the lysosome but remains associated with the lysosomal membrane. Inefficient translocation of the Tau fragments across the lysosomal membrane seems to promote formation of Tau oligomers at the surface of these organelles which may act as precursors of aggregation and interfere with lysosomal functioning.

MeSH Terms
Animals Autophagy Blotting, Western Cathepsin L/metabolism Cell Line, Tumor HSC70 Heat-Shock Proteins/metabolism Humans Lysosomes/metabolism Mice Microtubule-Associated Proteins/metabolism Models, Biological Mutation Peptide Fragments/chemistry,genetics,metabolism Phagosomes/metabolism Protein Binding Protein Multimerization Protein Transport Tauopathies/metabolism tau Proteins/chemistry,genetics,metabolism
Chemicals
HSC70 Heat-Shock Proteins Map1lc3b protein, mouse Microtubule-Associated Proteins Peptide Fragments tau Proteins Cathepsin L
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Wang Yipeng
Max-Planck-Unit for Structural Molecular Biology, Notkestrasse 85, 22607 Hamburg, Germany.
Martinez-Vicente Marta
Krüger Ulrike
Kaushik Susmita
Wong Esther
Mandelkow Eva-Maria
Cuervo Ana Maria
Mandelkow Eckhard
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Article Info
Journal
Human molecular genetics
Abbr.
Hum Mol Genet
ISSN
1460-2083
Published
2009-11-01
Epub
2009-00-04
Pages
4153-70
Language
English
Region
England
NLM ID
9208958
PMCID
PMC2758146
Subset
IM
Grants
NIA NIH HHS · AG031782 · United States
NINDS NIH HHS · NS038370 · United States
Analysis Services
Analysis Services

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