Heat-inducible hygromycin resistance in transgenic tobacco.
We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gene transfer. Heat-inducible synthesis of mRNA was shown by northern hybridization, and translation of this RNA into a functional protein was indicated by plant growth on hygromycin-containing media in a temperature-dependent fashion. One hour incubation at 40 degrees C per day, applied for several weeks, was sufficient to express the resistant phenotype in transgenic plants containing the chimaeric hs-hpt gene. These data suggest that the hygromycin resistance gene is functional and faithfully controlled by the soybean hs promoter. The suitability of these transgenic plants for selection of mutations that alter the hs response is discussed.
29 references, click to expand
-
Constitutive binding of yeast heat shock factor to DNA in vivo.Mol Cell Biol. 1988 Nov;8(11):5040-2 PMID: 3062378
-
The preferential translation of Drosophila hsp70 mRNA requires sequences in the untranslated leader.Cell. 1985 Oct;42(3):903-11 PMID: 4053186
-
Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene families.J Mol Biol. 1988 Feb 20;199(4):549-57 PMID: 3351943
-
Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium.EMBO J. 1985 Aug;4(8):1921-6 PMID: 16453629
-
Purified human factor activates heat shock promoter in a HeLa cell-free transcription system.J Biol Chem. 1988 Dec 25;263(36):19734-9 PMID: 3198647
-
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
-
A stable bifunctional antisense transcript inhibiting gene expression in transgenic plants.Proc Natl Acad Sci U S A. 1988 Jun;85(12):4300-4 PMID: 16593941
-
Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation.Cell. 1988 Sep 9;54(6):855-64 PMID: 3044613
-
A chimaeric hygromycin resistance gene as a selectable marker in plant cells.Plant Mol Biol. 1985 Sep;5(5):299-302 PMID: 24306921
-
Induction of sequence-specific binding of Drosophila heat shock activator protein without protein synthesis.Nature. 1987 Jun 25-Jul 1;327(6124):727-30 PMID: 3600771
-
Alterations of Endogenous Cytokinins in Transgenic Plants Using a Chimeric Isopentenyl Transferase Gene.Plant Cell. 1989 Apr;1(4):403-413 PMID: 12359893
-
Foreign genes in plants: transfer, structure, expression, and applications.Annu Rev Genet. 1988;22:421-77 PMID: 3071255
-
Mutations that induce the heat shock response of Drosophila.Cell. 1987 Dec 4;51(5):763-72 PMID: 3119225
-
Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.Science. 1986 Oct 24;234(4775):464-6 PMID: 17792019
-
Genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family.Mol Cell Biol. 1985 Dec;5(12):3417-28 PMID: 3939317
-
Binary Agrobacterium vectors for plant transformation.Nucleic Acids Res. 1984 Nov 26;12(22):8711-21 PMID: 6095209
-
Stable binding of Drosophila heat shock factor to head-to-head and tail-to-tail repeats of a conserved 5 bp recognition unit.Cell. 1989 Dec 1;59(5):797-806 PMID: 2590940
-
Isolation of mutations that act in trans to alter expression from a yeast hsp70 promoter.Mol Cell Biol. 1988 Aug;8(8):3423-31 PMID: 3145411
-
The heat-shock response.Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
-
An Arabidopsis thaliana cDNA clone encoding a low molecular weight heat shock protein.Nucleic Acids Res. 1989 Oct 11;17(19):7995 PMID: 2798141
-
Functional analysis of sequences required for transcriptional activation of a soybean heat shock gene in transgenic tobacco plants.EMBO J. 1987 May;6(5):1161-6 PMID: 16453761
-
Trimerization of a yeast transcriptional activator via a coiled-coil motif.Cell. 1989 Dec 1;59(5):807-13 PMID: 2686840
-
Artificial combination of two cis-regulatory elements generates a unique pattern of expression in transgenic plants.Proc Natl Acad Sci U S A. 1987 Dec;84(24):8986-90 PMID: 3480523
-
A simple and general method for transferring genes into plants.Science. 1985 Mar 8;227(4691):1229-31 PMID: 17757866
-
Regulatory domains of the Gmhsp17.5-E heat shock promoter of soybean.Mol Cell Biol. 1989 Aug;9(8):3457-63 PMID: 2796991
-
Heat shock proteins of higher plants.Proc Natl Acad Sci U S A. 1981 Jun;78(6):3526-30 PMID: 16593032
-
Characterization of two genes encoding small heat-shock proteins in Arabidopsis thaliana.Mol Gen Genet. 1989 Nov;219(3):365-72 PMID: 2482931
-
The function of plant heat shock promoter elements in the regulated expression of chimaeric genes in transgenic tobacco.Mol Gen Genet. 1989 Jun;217(2-3):246-53 PMID: 2770695
-
A family of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing.Gene. 1988 Oct 15;70(1):171-9 PMID: 2907323
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