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PMID: 19696344 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Formation of the first peptide bond: the structure of EF-P bound to the 70S ribosome.

Science (New York, N.Y.) ·Vol. 325 ·No. 5943 ·2009-08-21 ·Pages 966-70

Blaha G, Stanley RE, Steitz TA

Abstract

Elongation factor P (EF-P) is an essential protein that stimulates the formation of the first peptide bond in protein synthesis. Here we report the crystal structure of EF-P bound to the Thermus thermophilus 70S ribosome along with the initiator transfer RNA N-formyl-methionyl-tRNA(i) (fMet-tRNA(i)(fMet)) and a short piece of messenger RNA (mRNA) at a resolution of 3.5 angstroms. EF-P binds to a site located between the binding site for the peptidyl tRNA (P site) and the exiting tRNA (E site). It spans both ribosomal subunits with its amino-terminal domain positioned adjacent to the aminoacyl acceptor stem and its carboxyl-terminal domain positioned next to the anticodon stem-loop of the P site-bound initiator tRNA. Domain II of EF-P interacts with the ribosomal protein L1, which results in the largest movement of the L1 stalk that has been observed in the absence of ratcheting of the ribosomal subunits. EF-P facilitates the proper positioning of the fMet-tRNA(i)(fMet) for the formation of the first peptide bond during translation initiation.

MeSH Terms
Bacterial Proteins/chemistry,metabolism Binding Sites Crystallography, X-Ray Models, Molecular Peptide Chain Initiation, Translational Peptide Elongation Factors/chemistry,metabolism Protein Conformation Protein Structure, Tertiary RNA, Bacterial/chemistry,metabolism RNA, Messenger/chemistry,metabolism RNA, Transfer, Met/chemistry,metabolism Ribosomal Proteins/metabolism Ribosome Subunits, Large, Bacterial/metabolism Ribosome Subunits, Small, Bacterial/metabolism Ribosomes/metabolism Thermus thermophilus/chemistry,metabolism
Chemicals
Bacterial Proteins Peptide Elongation Factors RNA, Bacterial RNA, Messenger RNA, Transfer, Met Ribosomal Proteins fMet-tRNA(fMet) factor EF-P ribosomal protein L1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blaha Gregor
Department of Molecular Biophysics, Yale University, New Haven, CT 06520, USA.
Stanley Robin E
Steitz Thomas A
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1175800
Published
2009-08-21
Pages
966-70
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC3296453
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · P01 GM022778 · United States
NIGMS NIH HHS · P01 GM022778-36 · United States
NIGMS NIH HHS · GM22778 · United States
Databases
PDB
3HUW, 3HUX, 3HUY, 3HUZ
Corrections
CommentIn
PMID: 19833922
External Links
PubMed source DOI: 10.1126/science.1175800 PMC Full Text View journal details
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