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PMID: 19706478 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Study of free oligosaccharides derived from the bacterial N-glycosylation pathway.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 106 ·No. 35 ·2009-09-01 ·Pages 15019-24

Nothaft H, Liu X, McNally DJ, Li J, Szymanski CM

Abstract

The food-borne pathogen Campylobacter jejuni is one of the leading causes of bacterial gastroenteritis worldwide and the most frequent antecedent in neuropathies such as the Guillain-Barré and Miller Fisher syndromes. C. jejuni was demonstrated to possess an N-linked protein glycosylation pathway that adds a conserved heptasaccharide to >40 periplasmic and membrane proteins. Recently, we showed that C. jejuni also produces free heptasaccharides derived from the N-glycan pathway reminiscent of the free oligosaccharides (fOS) produced by eukaryotes. Herein, we demonstrate that C. jejuni fOS are produced in response to changes in the osmolarity of the environment and bacterial growth phase. We provide evidence showing the conserved WWDYG motif of the oligosaccharyltransferase, PglB, is necessary for fOS release into the periplasm. This report demonstrates that fOS from an N-glycosylation pathway in bacteria are potentially equivalent to osmoregulated periplasmic glucans in other Gram-negative organisms.

MeSH Terms
Campylobacter jejuni/chemistry,metabolism Glycosylation Hexosyltransferases/genetics,metabolism Mass Spectrometry Mutation/genetics Oligosaccharides/chemistry,metabolism Osmotic Pressure Periplasm/metabolism Polysaccharides/metabolism Transcription, Genetic
Chemicals
Oligosaccharides Polysaccharides Hexosyltransferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nothaft Harald
Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada K1A 0R6.
Liu Xin
McNally David J
Li Jianjun
Szymanski Christine M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0903078106
Published
2009-09-01
Epub
2009-00-14
Pages
15019-24
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2736414
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0903078106 PMC Full Text View journal details
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