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PMID: 1972856 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mRNA encoding the scrapie agent protein is present in a variety of non-neuronal cells.

Acta neuropathologica ·Vol. 80 ·No. 1 ·1990-00-00 ·Pages 1-6

Brown HR, Goller NL, Rudelli RD, Merz GS, Wolfe GC, Wisniewski HM, Robakis NK

Abstract

PrP 27-30, a unique protease-resistant protein associated with scrapie infectivity, derives from the proteolytic cleavage of a larger precursor encoded by a host gene. To identify sites of PrP biosynthesis, in situ hybridization was done using cloned PrP cDNA as a probe. In rodent brain, PrP mRNA was expressed in neurons, ependymal cells, choroid plexus epithelium, astrocytes, pericytes, endothelial cells and meninges of both scrapie-infected and uninfected animals. PrP mRNA was also detected in vitro in isolated brain microglia cells. Pulmonary cells and heart muscle cells contained high levels of this mRNA. Hybridization was not detected in spleen, confirming earlier RNA blot experiments indicating extremely low levels of PrP mRNA in this tissue. Results indicate that PrP mRNA is a normal component in a variety of non-neuronal tissues and may explain the origin of the amyloid plaques present in the subependymal region of scrapie-infected brain.

MeSH Terms
Animals Brain/metabolism,pathology DNA/metabolism Gene Expression Regulation Mice Mice, Inbred C57BL Nucleic Acid Hybridization PrP 27-30 Protein RNA, Messenger/genetics,metabolism Scrapie/metabolism,pathology Viral Proteins/genetics,metabolism
Chemicals
RNA, Messenger Viral Proteins PrP 27-30 Protein DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Brown H R
New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314.
Goller N L
Rudelli R D
Merz G S
Wolfe G C
Wisniewski H M
Robakis N K
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Article Info
Journal
Acta neuropathologica
Abbr.
Acta Neuropathol
ISSN
0001-6322
DOI
10.1007/BF00294214
Published
1990-00-00
Pages
1-6
Language
English
Region
Germany
NLM ID
0412041
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00294214 View journal details
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