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PMID: 197530 Published · ppublish English Journal Article

Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 3052-5

Rubinstein M, Stein S, Gerber LD, Udenfriend S

Abstract

Rat beta-lipotropin was isolated from 40 anterior pituitaries using high efficiency chromatography columns and sensitive fluorometric methods. The beta-lipotropin was characterized as to molecular weight, amino acid composition, and generation of opioid activity by trypsinization. Other opioid peptides, as well as a precursor to opioid peptides with a molecular weight larger than that of beta-lipotropin, were observed in this tissue.

MeSH Terms
Amino Acids/analysis Animals Endorphins/isolation & purification Enkephalins/isolation & purification Male Molecular Weight Pituitary Gland/analysis Pituitary Gland, Anterior/analysis Rats Receptors, Opioid/metabolism Trypsin/metabolism beta-Lipotropin/analysis,isolation & purification,metabolism
Chemicals
Amino Acids Endorphins Enkephalins Receptors, Opioid beta-Lipotropin Trypsin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rubinstein M
Stein S
Gerber L D
Udenfriend S
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.7.3052
Published
1977-07-00
Pages
3052-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431409
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.7.3052 PMC Full Text View journal details
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