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PMID: 1976512 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Thymic selection defines multiple T cell receptor V beta 'repertoire phenotypes' at the CD4/CD8 subset level.

The EMBO journal ·Vol. 9 ·No. 11 ·1990-11-00 ·Pages 3641-8

Singer PA, Balderas RS, Theofilopoulos AN

Abstract

We describe here the use of a sensitive and accurate multiprobe V beta RNase protection assay in characterizing the expression levels of 17 V beta genes in separated CD4+ and CD8+ subsets of selected mouse strains. The IE-reactive V beta genes (V beta s 11, 12, 5.1 and 16) showed various patterns of skewed subset expression in different strains, suggesting additional influences of IA, class I, and non-MHC genes in the selection process. Clonal deletion of V beta 11- and V beta 12-bearing T cells, among others, was skewed strongly towards the CD4+ subset in many IE+ mouse strains, supporting the notion that negative selection can cause incomplete, subset biased, V beta clonal deletions. Broad analysis in separated CD4+ and CD8+ subsets gave improved resolution of V beta repertoire selection, and revealed significant strain and/or subset specific skewing for additional V beta genes; with consistent bias towards higher expression of V beta 7 and V beta 13 in the CD8+ subset, and V beta 15 in the CD4+ subset of most mouse strains. The influence of diverse non-MHC ligands in V beta repertoire selection was further illustrated by the identification of unique V beta repertoires for six different MHC-identical (H2k) strains. Such polymorphisms in TCR repertoire expression may help to define better disease susceptibility phenotypes.

MeSH Terms
Animals CD4-Positive T-Lymphocytes/physiology Gene Expression Gene Rearrangement, beta-Chain T-Cell Antigen Receptor Histocompatibility Antigens Class II/physiology Mice Mice, Inbred Strains RNA Probes RNA, Messenger/metabolism Receptors, Antigen, T-Cell/genetics Receptors, Antigen, T-Cell, alpha-beta Spleen/cytology T-Lymphocyte Subsets/physiology Thymus Gland/cytology
Chemicals
Histocompatibility Antigens Class II I-E-antigen RNA Probes RNA, Messenger Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Singer P A
Immunology Department/IMM3 Scripps Clinic and Research Foundation, La Jolla, CA 92037.
Balderas R S
Theofilopoulos A N
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33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-11-00
Pages
3641-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552117
Subset
IM
Grants
NIAMS NIH HHS · AR31203 · United States
PHS HHS · ASR39555 · United States
NIDDK NIH HHS · DK40751 · United States
External Links
PubMed source PMC Full Text View journal details
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